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  • New strategy to reveal the black seeds of melanomas (cancer stem cells) with their vulnerable characteristics at cellular and molecular levels

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Poster Presentation
New strategy to reveal the black seeds of melanomas (cancer stem cells) with their vulnerable characteristics at cellular and molecular levels
  1. Beatrix Kotlan1,6,
  2. Gabriella Liszkay2,7,8,
  3. Gyorgy Naszados3,
  4. Zoltan Dolescall4,
  5. Laszlo Toth5,
  6. Laszlo Gobor5,
  7. Istvan N Vamosy5,
  8. Andras Szollar5,
  9. Szabolcs Horvath6,
  10. Klara Eles6,
  11. Orsolya Csuka4,7,
  12. Miklos Kasler7,8,
  13. Maria Godeny3,7,8 and
  14. Francesco M Marincola9
  1. Aff1 grid.419617.c0000000106678064Molecular Immunology and ToxicologyNational Institute of Oncology Budapest Hungary
  2. Aff2 grid.419617.c0000000106678064OncodermatologyNational Institute of Oncology Budapest Hungary
  3. Aff3 grid.419617.c0000000106678064Radiological DiagnosticsNational Institute of Oncology Budapest Hungary
  4. Aff4 grid.419617.c0000000106678064PathogeneticsNational Institute of Oncology Budapest Hungary
  5. Aff5 grid.419617.c0000000106678064OncosurgeryNational Institute of Oncology Budapest Hungary
  6. Aff6 grid.419617.c0000000106678064Surgical and Molecular TumorpathologyNational Institute of Oncology Budapest Hungary
  7. Aff7 grid.419617.c0000000106678064Board of DirectorsNational Institute of Oncology Budapest Hungary
  8. Aff8 Univ Med Pharm Tirgu Mures Romania
  9. Aff9 grid.467063.00000000403974222SIDRA Medical and Research Center Doha Qatar

https://doi.org/10.1186/2051-1426-1-S1-P160

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    Meeting abstracts

    Objectives

    A suitable approach to select cancer stem cells (CSC), the black seed of melanomas would enable their characterization and elimination.

    Methods

    Cancerous tissues from primary and metastatic lesions of patients with malignant melanomas (n=150) were investigated by cell cultures and molecular genetics. Double labelled cells were sorted by BD FACSAvia Sorter. Gene expression analysis by Real Time PCR (MYiQTM, BIO-RAD) and RNA microarray (Agilent) has been performed (n=48).

    Results

    90% of the cell cultures grew and cancer initiating cells could be IF FACS sorted (0,1% - 1 %). Colocalisation of unique GD3 sialilated glycosphingolipids and antiCD20 binding was proved. Characteristic growth pattern, spheroid forming, CSC markers (e.g. CD133, Nestin, ABCB5, CD20 and unique GD3) was observed (Figure 1.a,b,c,d). We found enhanced gene expression of CXCR4 and other markers correlated to metastatic potential, clinical outcome and CSC presence. High throughput gene expression microarray analysis of RNA preparations of punch biopsies and CSC outgrowth are compared.

    Figure

    Figure 1

    Conclusion

    Unique GD3 sialilated glycosphingolipids with colocalised CD20 proved to be selection markers for CSC in metastatic melanomas. Our strategy paves the way for detection and characterization of cancer stem cells and provides material for therapeutic developments to eliminate the black seeds of melanomas.

    Acknowledgments

    Harry J Lloyd Charitable Trust Melanoma Research Award.