Methods

We applied flow-cytometer analysis of PBMCs harvested before treatment from patients (n=43) enrolled in a small randomized phase II study of docetaxel alone (n=20) or in combination with PANVAC^TM-V (Vaccinia) and PANVAC^TM-F (Fowlpox) encoding for the tumor-associated antigens CEA and MUC-1, along with a TRIad of COstimulatory Molecules (B7-1, ICAM-1, and LFA-3; called TRICOM) (n=23). As criterion 1, we analyzed the frequency standard immune subsets, i.e. CD4, CD8, NK, Treg, MDSC, and their ratios. As criterion 2, we measured phenotypes indicating immune function, i.e. central memory T lymphocytes, T cells expressing at ≥2 suppressive markers among CTLA-4, PD1, TIM3, and 2B4, CD49d^- Tregs, lin^- MDSCs, CD56^brCD16^- NK cells, and their ratios. An immunoscore was generated based on the analysis of tertiles. Log-Rank Kaplan Meier analysis was applied to evaluate differences of PFS between patients with low- and- high immunoscore.