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1002 Circulating myeloid states induced with anti-PD-1 and GM-CSF combination therapy are associated with treatment resistance in human biliary cancer
  1. Bridget Keenan1,
  2. Elizabeth McCarthy1,
  3. Arielle Ilano1,
  4. Diamond Luong1,
  5. Hai Yang1,
  6. Li Zhang1,
  7. Kathryn Allaire1,
  8. Zenghua Fan1,
  9. Tony Li2,
  10. David Lee2,
  11. Yang Sun1,
  12. Alexander Cheung1,
  13. Hewitt Chang1,
  14. Brandon Chen1,
  15. Brenna Sheldon1,
  16. Robin Kelley1,
  17. Chun Jimmie Ye1 and
  18. Lawrence Fong1
  1. 1University of California San Francisco, San Francisco, CA, United States
  2. 2University of Washington, Seattle, WA, United States


Background Advanced biliary tract cancers (BTC) have a poor prognosis and low rates of response to immune checkpoint inhibition (CPI), with overall response rates ranging from 3-13%.1-3 The BTC tumor microenvironment contains immunosuppressive immune populations, desmoplastic stroma, and a paucity of tumor infiltrating effector T cells4,5, but the role of circulating immune cells in response and resistance to CPI is less well-characterized. We aimed to explore circulating immune cells from BTC patients treated with CPI to understand immunotherapy response and resistance.

Methods We used multiplexed multi-omic single cell RNA sequencing and cell surface proteomics (CITEseq) to profile >400,000 circulating immune cells in BTC patients receiving anti-PD-1 with and without GM-CSF as part of a phase II clinical trial (NCT02703714)6, as well as from age and gender-matched healthy donors. We also evaluated a subset of tumor tissues from BTC patients to investigate the spatial organization of immune cell populations.

Results Using CITEseq, we identified several unique CD14+ monocyte sub-populations in BTC patients’ circulation compared to healthy donors. In the circulation of patients with BTC tumors that are CPI-resistant, there was an increased frequency of “CD14CTX”, a monocyte sub-population expressing high levels of immunosuppressive cytokines and chemotactic molecules, following anti-PD-1 monotherapy. CD14CTX can directly suppress CD4+ T cells and induce SOCS3 expression in CD4+ T cells, rendering them functionally unresponsive. Analogous tumor-associated macrophage populations could also be found within tumor tissues. Consistent with the pleiotropic effects of GM-CSF, we saw changes in the frequency and genomic programs of multiple myeloid populations, including dendritic cells and monocytes, when GM-CSF was combined with anti-PD-1. Within one week of GM-CSF combination therapy, monocyte-derived dendritic cells were induced in the circulation of BTC patients and associated with pro-inflammatory signals, including upregulation of MHC I and II in monocytes and dendritic cells.

Conclusions These results demonstrate the capacity of CPI plus GM-CSF combination therapy to induce monocyte states that both foster and inhibit adaptive immunity. Monocytes arising after anti-PD-1 treatment induced T cell paralysis in a subset of patients with CPI-resistant tumors, as a distinct mode of tumor-mediated immunosuppression. GM-CSF demonstrated the potential to enhance both pro-inflammatory and antigen-processing and presentation-related programs within myeloid cells. These results highlight the importance of identifying pharmacodynamic markers of immune response and targets for combination immunotherapy to overcome CPI resistance in BTC.

Trial Registration NCT02703714


  1. Ueno M, et al. Nivolumab alone or in combination with cisplatin plus gemcitabine in Japanese patients with unresectable or recurrent biliary tract cancer: a non-randomised, multicentre, open-label, phase 1 study. Lancet Gastroenterol Hepatol 2019;4:611-621.

  2. Piha-Paul SA, et al. Efficacy and safety of pembrolizumab for the treatment of advanced biliary cancer: Results from the KEYNOTE-158 and KEYNOTE-028 studies. Int J Cancer 2020.

  3. Kim RD, et al. A Phase 2 multi-institutional study of nivolumab for patients with advanced refractory biliary tract cancer. JAMA Oncol 2020;6: 888–894.

  4. Rizvi S, Khan SA, Hallemeier CL, Kelley RK & Gores GJ. Cholangiocarcinoma – evolving concepts and therapeutic strategies. Nat Rev Clin Oncol 2018;15, 95–111.

  5. Zhang M, et al. Single cell transcriptomic architecture and intercellular crosstalk of human intrahepatic cholangiocarcinoma. Journal of Hepatology 2020.

  6. Kelley RK, et al. Phase II trial of pembrolizumab (PEM) plus granulocyte macrophage colony stimulating factor (GM-CSF) in advanced biliary cancers (ABC). Journal of Clinical Oncology 2018;36, 386–386.

Ethics Approval Peripheral blood mononuclear cells were obtained from patients (per UCSF institutional review board (IRB) #15-18420) from the clinical trial #NCT02703714. Tumor samples were collected from patients biopsied as part of the clinical trial and from patients undergoing standard-of-care resections and consented under the UCSF Hepatobiliary Tissue Bank and Registry (IRB #12-09576). Healthy donor PBMCs were collected from age and gender-matched healthy donors as part of the Cancer Immunotherapy Biobanking protocol and the Immune Cell Census (IRB #15-16385 and #19-27147, respectively). Informed consent was obtained from all patients for participation in the listed trials and for use of blood and tumor samples in research studies.

Consent None of the patient data in this abstract is identifiable; IRB information included as above.

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