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118 Delineation of spatial tissue signatures of immunotherapy response groups in non-small cell lung cancer (NSCLC)
  1. Arutha Kulasinghe1,
  2. James Monkman1,
  3. Honesty Kim2,
  4. Aaron Mayer2,
  5. Ahmed Mehdi1,
  6. Marie Cumberbatch3,
  7. Milan Bhagat3,
  8. Fabian Schneider4,
  9. Jeppe Thaagard4,
  10. James Mansfield4,
  11. Rahul Ladwa5,
  12. Scott Mueller6,
  13. Mark Adams7 and
  14. Kenneth O’byrne5
  1. 1The University of Queensland, Brisbane, Australia
  2. 2Enable Medicine, California, CA, USA
  3. 3Tristar Technologies, London, DC, UK
  4. 4Visiopharm, Horsholm, Denmark
  5. 5The Princess Alexandra Hospital, Brisbane, Australia
  6. 6University of Melbourne, Melbourne, Australia
  7. 7Queensland University of Technology, Brisbane, Australia


Background Lung cancers remain the leading cause of cancer related mortality and have a poor 5-year survival. Immunotherapies have led to durable benefit in a cohort of non-small cell lung cancer (NSCLC) patients. Identifying those patient likely to achieve benefit remains a clinical unmet need. Whilst predictive biomarkers such as PD-L1 and tumour mutation burden (TMB) have shown utility, the underlying tumour-immune biology is unlikely represented. The composition and activation status of the cellular milieu contained within the tumour microenvironment (TME) is becomingly increasingly recognised as a driving factor dictating response to immunotherapies.

Methods In this study, we employed multiplex IHC (mIHC), and digital spatial profiling (DSP) to capture the targeted immune proteome and transcriptome of tumour and TME compartments from ICI-treated (n=41) and standard of care (n=47) NSCLC patient cohorts. Oncotopix® Discovery was also used to analyse the highplex imagery. The analysis pipeline consisted of tissue segmentation (tumor, stroma, necrosis, etc), nuclear detection using a deep-learning algorithm for DAPI, a threshold-based cellular phenotyping step, and spatial analyses.

Results Patients sensitive to ICI therapy expressed higher levels of IL2 receptor alpha (CD25, p=0.028) within the tumour compartments, which corresponded with increased IL2 mRNA (p=0.001) within their stroma. IL2 mRNA levels within the stroma positively correlated with the expression of pro-apoptotic markers cleaved caspase 9 (p=2e-5) and BAD (p=5.5e-4) and negatively with levels of memory T cells (CD45RO) (p=7e-4). Immuno-inhibitory markers CTLA-4 (p=0.021) and IDO-1 (p=0.023) were supressed in ICI-responsive patients. Tumour CD44 (p=0.02) was depleted in the response group and corresponded inversely with higher stromal expression of one of its ligands, SPP1(osteopontin, p=0.008). Cox survival analysis indicated tumour CD44 expression was associated with poorer prognosis (HR=1.61, p=0.01), consistent with its depletion in ICI sensitive patients. The SOC cohort paralleled similar roles for immune checkpoints and pro-apoptotic markers, with LAG3 (HR=3.81, p=0.04) indicating poorer outcome, and BIM (HR=0.16, p=0.014) with improved outcome.

Conclusions Through multi-modal approaches, we have dissected the characteristics of NSCLC treatment groups and provide evidence for the role of several markers including IL2, CD25, CD44 and SPP1 in the efficacy of current generations of ICI therapy. The signatures are being validated in prospective larger cohort studies.

Consent This study has Queensland University of Technology (QUT) Human Research Ethics Committee Approval (UHREC #2000000494).

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