Article Text

Download PDFPDF

587 AMADEUS trial: tumor agnostic pre- and on-treatment biomarkers of response to nivolumab plus ipilimumab correlate with on-treatment tumoral T cell infiltration
  1. Farah Alayli1,
  2. Kwame Okrah1,
  3. Apostolia Tsimberidou2,
  4. Alexandra Drakaki3,
  5. Danny Khalil4,
  6. Saad Khan5,
  7. Stephen Hodi6,
  8. David Oh7,
  9. Meelad Amouzgar1,
  10. Shikha Guatam1,
  11. Robin Kageyama1,
  12. Shannon Pfeiffer1,
  13. Stefanie Meier1,
  14. Christopher Cabanski1,
  15. Diane Da Silva1,
  16. Dinesh Kumar1,
  17. Sandra Santulli-Marotto1,
  18. Michael Tetzlaff7,
  19. Wai Chin Foo2,
  20. Travis Hollman4,
  21. Yanyun Li4,
  22. Mathew Adamow4,
  23. Philip Wong4,
  24. Marko Spasic1,
  25. Richard Chen8,
  26. Samantha Bucktrout1,
  27. Justin Fairchild1,
  28. Lisa Butterfield1,
  29. Theresa LaVallee1,
  30. Lacey Padron1,
  31. Lisa Salvador9,
  32. Jill O’Donnell-Tormey10,
  33. Ute Dugan1 and
  34. Padmanee Sharma2
  1. 1Parker Institute for Cancer Immunotherapy, San Francisco, United States
  2. 2The University of Texas MD Anderson Cancer Center, Houston, TX, United States
  3. 3University of California, Los Angeles, Los Angeles, United States
  4. 4Memorial Sloan Kettering Cancer Center, New York, United States
  5. 5Stanford University, Stanford, United States
  6. 6Dana-Farber Cancer Institute, Boston, United States
  7. 7University of California, San Francisco, San Francisco, United States
  8. 8Personalis Inc., Menlo Park, CA, United States
  9. 9Bristol Myers Squibb, Princeton, NJ, United States
  10. 10Cancer Research Institute, New York, United States


Background Although immune checkpoint inhibitors (ICI) are efficacious in some patients with cancer, many do not benefit. Identifying pre- and on-treatment biomarkers correlating with clinical outcomes is essential for better treatment strategies. High pre- and on-treatment recruitment of CD8+ T cells into the tumor are associated with improved outcomes following ICI therapy. We report orthogonal biomarker results from the AMADEUS study, an all-comer solid tumors trial, in which patients were treated with nivolumab (NIVO) with or without ipilimumab (IPI) based on the proportion of pretreatment tumoral CD8+ T cells.

Methods AMADEUS is a prospective, non-randomized, multicenter study that enrolled 79 patients with various metastatic solid tumors. The frequency of pretreatment tumoral CD8+ T cells was measured by a CLIA-certified immunohistochemistry (IHC) assay. Patients with ≥15% tumoral CD8 received NIVO monotherapy (CD8 high) and those with <15% tumoral CD8 received NIVO+IPI (CD8 low). Pre- and on-treatment tumor and blood samples were collected for longitudinal multi-omic biomarker analysis. Bulk RNA/DNA sequencing and high dimensional imaging technologies were used to analyze the tumor biopsies. Peripheral blood analyses included mass cytometry time of flight (CyTOF) for broad immune profiling, and a high parameter flow cytometry panel for T cell specific phenotypic evaluation.

Results In the CD8 low arm, on-treatment conversion from CD8 low to high (≥15%) was associated with a trend towards improved clinical benefit (p = 0.0582), whereas pretreatment tumoral CD8 T cell frequency was not correlated with clinical benefit. Analysis of tumor bulk mRNA sequencing yielded pan-tumor gene signatures of response and resistance to NIVO+IPI. Responders had elevated expression of genes relating to IFNg and JAK/STAT signaling, whereas glycolysis pathway genes and MYC targets dominated in the non-responders. Analysis of pretreatment peripheral blood immune cell populations demonstrated a significantly higher frequency of T cells with a stem cell progenitor-like phenotype (TCF1+) (p = 0.0179) in the non-responders compared to the responders. On-treatment, elevated frequencies of circulating gdT cells, NK cells, as well as enhanced activation and proliferation of effector and central memory conventional T cells were associated with response to ICI therapy.

Conclusions In this study, increased tumoral infiltration of CD8+ T cells on-treatment (≥15%) was associated with a trend towards improved clinical benefit (p = 0.0582). Using multi-omic analysis of pre- and on-treatment tissue and blood samples, we identified additional composite tumor agnostic biomarkers correlating with response. These biomarkers warrant further investigation for patient stratification and identification of novel drug targets to overcome ICI resistance.

Acknowledgements We extend our gratitude to the patients, their families, the clinical investigators, and their site staff members who made this trial possible. We would also like to thank Fizza Hussain and Carri Browne at Parker Institute for Cancer Immunotherapy (PICI) for operations leadership of the trial. We thank Bristol Myers Squibb (BMS) for collaboration and study drugs. The study was funded by Cancer Research Institute (CRI), BMS, and PICI.

Trial Registration ClinicalTrials. gov (NCT03651271)

Ethics Approval The study was approved by MD Anderson Cancer Center IRB, Approval Number: 2017-0446

Consent All participants provided written informed consent before enrollment

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.