Article Text

Download PDFPDF

1003 TIM-3+ natural killer cell dysfunction is driven by galectin-9 in head and neck squamous cell carcinoma
  1. Juncheng Wang1,
  2. Housaiyin Li1,
  3. Aditi Kulkarni1,
  4. Jennifer Anderson2,
  5. Lidia Arantes3,
  6. Hridesh Banerjee1,
  7. Lawrence P Kane1,
  8. Xin Zhang4,
  9. Tullia Bruno1,
  10. Riyue Bao2,
  11. Robert Ferris2 and
  12. Lazar Vujanovic1
  1. 1University of Pittsburgh, Pittsburgh, PA, USA
  2. 2UPMC Hillman Cancer Center, Pittsburgh, PA, USA
  3. 3Barretos Cancer Hospital, Barretos, SP, Brazil
  4. 4Xiangya Hospital, Changsha, China
  • Journal for ImmunoTherapy of Cancer (JITC) preprint. The copyright holder for this preprint are the authors/funders, who have granted JITC permission to display the preprint. All rights reserved. No reuse allowed without permission.


Background Natural killer (NK) cells are innate effector lymphocytes that play a central role in anti-tumor immunity. Their activation states are regulated by an interplay of activating and inhibitory surface receptors, including T-cell immunoglobulin and mucin-domain containing molecule 3 (TIM-3), an immune checkpoint receptor (ICR) that is expressed on terminally-differentiated NK cells. The role TIM-3 plays in the context of NK cell-mediated immunosurveillance remains evasive, partly because TIM-3 signaling is modulated by four ligands: galectin-9, phosphatidylserine, HMGB1 and CEACAM-1.

Methods Single-cell RNA sequencing and flow cytometry were implemented to study the prevalence, phenotypes and functional differences of TIM-3+ NK cells in head and neck squamous cell carcinoma (HNSCC) patient tumors and blood. In vitro killing and proliferation assays were used to evaluate whether the four TIM-3 ligands differentially modulate TIM-3+ NK cell functions, and whether disruption of TIM-3/ligand interaction can enhance NK cell-mediated anti-tumor effector mechanisms. Finally, TCGA survival analysis and digital spatial profiling were employed to study the potential impact of etiology-associated differences on HNSCC patient outcomes.

Results We demonstrate that TIM-3 is the dominant NK cell ICR, that it marks NK cells with enhanced effector potential in blood and tumors, and that galectin-9 is the only TIM-3 ligand that consistently suppresses NK cell cytotoxic and proliferative capacity. Galectin-9-induced effects on cytotoxicity can be abrogated using the clinical-grade anti-TIM-3 blocking antibody, MBG453. Clinically, high intratumoral TIM-3+ NK cell gene signature associates with worse outcome in HPV+, but not HPV- HNSCC patients. This may be due to higher intratumoral galectin-9 protein expression in HPV+ HNSCC lesions.

Conclusions Our data stress the importance and complexity of TIM-3 in the context of NK cells and suggest that targeting the TIM-3/galectin-9 pathway may be a cogent immunotherapeutic strategy to reinvigorate NK cell effector function in HPV+ HNSCC patients.

Acknowledgements We thank Merida Serrano, Amy Cuda and Denise Kroll for assistance with patient sample procurement. This research utilized the Hillman Cancer Center Flow Cytometry Core Facility, supported in part by award P30 CA047904 (RLF). This research was supported in part by the University of Pittsburgh Center for Research Computing, RRID:SCR_022735, through the resources provided. Specifically, this work used the HTC cluster, which is supported by NIH award number S10OD028483.

Ethics Approval Peripheral blood and tumor tissues from treatment-naïve HNSCC patients were collected with their written consent in accordance with the Declaration of Helsinki, under the University of Pittsburgh Cancer Institute Review Board–approved protocol (99-069).

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.