Article Text
Abstract
Background Adoptive transfer of autologous T cells derived from tumor fragments cultured in IL-2 have been successful in treating melanomas and select epithelial cancers.1 2 However, tumor-reactive T cells are a small percentage of all TIL while most are bystander cells.3 Identification of neoantigen-reactive TIL for treatment involves time-intensive testing of TIL cultures against patient-specific candidate neoantigens, resulting in selection of TIL at the fragment rather than cell level and relying on tumor sequencing and testing of patient-specific reagents.4 5
Methods In this study, we cultured multiple fragments from resected tumors together in GREX100 flasks with IL-2 (n=14 patients). In 3 weeks, these cultures yielded a median of 3.09e8 (1.4e8-6e8) TIL. The large cell yield allowed the study of cell surface marker expression for sorting tumor-reactive TIL by FACS.
To identify candidate markers, we performed single-cell RNA-, CITE- and T cell receptor (TCR)-sequencing on TIL from a human rectal cancer lung metastasis. We cloned and expressed high frequency CD8 TCRs in peripheral blood lymphocytes (PBL) and screened them for tumor reactivity. Twelve TCRs showed functional reactivity to tumor neoantigens and 3 TCRs were reactive to CMV/EBV/Flu peptides (indicating true bystanders). Projecting these relevant TCRs onto the transcriptomic map indicated that cells with tumor-reactive TCRs clustered separately from bystanders (figure 1A). Antibody expression analysis showed CD103 as the top marker for reactive cells and CD31 for bystander cells (figure 1B).
Results Applying these findings to 4 prospective patients with metastatic epithelial cancer, we analyzed the expression of CD103 and CD31 within CD8+ TIL from pooled fragment cultures. The percentage of CD103+CD31- cells in these patients ranged from 2.12% to 29.6%. We sorted TIL according to CD103/CD31 expression and performed a rapid expansion protocol (REP). The CD103+CD31- population had a median expansion of 833.5 (285–1081) fold. Subsequent screens against candidate neoantigens demonstrated enriched reactivity among CD103+CD31- CD8+ TIL in all 4 patients compared to CD103- and CD31+ cells. In one patient, reactivity (% 4–1BB+ TIL following specific antigenic stimulation [5]) increased from 6% in the bulk, unsorted and un-REPed cultures to 25% among the REPed CD103+CD31- cells.
Conclusions Taken together, this study can help identify reactive TIL from bulk cultures of tumor fragments without requiring patient-specific reagents for testing. Studies are ongoing in scalability of this approach to generate high numbers of these TIL for patient treatment. If successful, this approach has the potential to decrease the time between resection to treatment.
References
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Ethics Approval This study was approved by National Institutes of Health (NIH) Internal Review Board (IRB); approval number 03-C-0277
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