Article Text

Download PDFPDF

567 Merkel cell carcinomas are infiltrated by tumor-associated macrophages that counteract intratumoral CD8 T-cell benefit and correlate with resistence to PD-1 pathway blockade
  1. Shira Tabachnick-Cherny1,
  2. Thomas Pulliam1,
  3. Haroldo Rodriguez Chevez1,
  4. Xinyi Fan2,
  5. Daniel S Hippe2,
  6. Daniel C Jones2,
  7. Ata Moshiri3,
  8. Kimberly S Smythe2,
  9. Rima Kulikauskas1,
  10. Lisa Zaba4,
  11. Kelly Paulson5 and
  12. Paul Nghiem1
  1. 1University of Washington, Seattle, WA, USA
  2. 2Fred Hutchinson Cancer Center, Seattle, WA, USA
  3. 3New York University, New York, NY, USA
  4. 4Stanford University School of Medicine, Palo Alto, CA, USA
  5. 5Swedish Cancer Institute, Edmonds, WA, USA

Abstract

Background PD-(L)1 blockade has transformed treatment for advanced Merkel cell carcinoma (MCC), with over 50% of patients initially responding to therapy. However, the need to understand the underlying biology of responses and explore alternative treatments for non-responsive patients remains unmet. MCC is a highly immunogenic skin cancer that is caused by the Merkel cell polyomavirus in ~80% of cases with remaining cases due to UV exposure. Both etiologies result in high immunogenicity due to viral antigens or UV-neoantigens. Prior studies of MCC immuno-biology have mainly focused on CD8 T-cells and their exhaustion status. However, the role of innate immunity, particularly myeloid cells in MCC immune evasion, has not been well studied. We sought to investigate the types of myeloid cells in MCC and their association with outcomes to PD-(L)1 blockade.

Methods Myeloid cell profiling in MCC tumors was conducted using single-cell RNA sequencing (scRNAseq) of dissociated tumors from 9 patients. MCC tumors collected from 54 patients prior to anti-PD-(L)1 treatment were then used to identify myeloid cells via multiplex-IHC and evaluate their association with response.

Results scRNAseq analysis showed that tumor-associated macrophages (TAMs) are the main myeloid component within MCC tumors. TAMs in MCC express an immunosuppressive gene signature characteristic of monocytic myeloid derived suppressor cells1 and express targetable immune checkpoint molecules, including PD-L1 and LILRB receptors, that are not present on tumor cells. Analysis of 54 pre-immunotherapy tumor samples showed that a subset of TAMs (CD163+, CD14+, S100A8+) correlated with CD8 T-cell infiltration, despite their presumed immunosuppressive role. Stratifying tumor samples based on CD8 T cell levels and measuring the TAMs-to-CD8 T-cell ratio (TAM/CD8) revealed a suppressive relationship between TAMs and CD8 T cells. A higher TAM/CD8 ratio among high CD8 tumors was associated with resistance to PD-(L)1 blockade treatment, and the ratio was a far better predictor of initial response than CD8 T-cell level alone (ROC analysis: TAM/CD8 ratio AUC = 0.96, p=0.008; CD8% AUC = 0.60, p=0.544). Progression free survival analysis of the 54-patient validation cohort also showed that a higher TAM/CD8 ratio predicted earlier disease progression in PD-(L)1-treated patients (HR: 1.24 per 2-fold increase, p = 0.0074).

Conclusions Immunosuppressive activity of TAMs in MCC tumors may counteract the benefit of intratumoral CD8 T-cells, reducing the likelihood of response to treatment. This study suggests that immunotherapy-refractory MCC may be an appropriate setting in which to explore myeloid-targeted therapies in clinical trials.

Acknowledgements We acknowledge Dr. Cecilia Yeung, PI of the Clinical Trials Pathology Lab (CTP) at Fred Hutchinson Cancer Center for her advice and determination of appropriate localization and staining pattern of each multiplex IHC marker. We would also like to thank CLIA lab, including members from the Experimental Histopathology lab and from the McGarry Houghton’s team for the technical support of the TMA build, antibody optimizations, and samples staining and imaging. We would like to thank MCC patients and their families for agreeing to donate specimens and support our research progress.

References

  1. Veglia, F., Sanseviero, E. & Gabrilovich, D.I. Myeloid-derived suppressor cells in the era of increasing myeloid cell diversity. Nat Rev Immunol 2021; 21, 485–498

Ethics Approval The study was approved by Fred Hutchinson Cancer Center Ethics Board, approval number 6585.

http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.