Article Text

Download PDFPDF

704 Biological activity of FLX475, an oral CCR4 antagonist, as monotherapy and in combination with pembrolizumab in advanced cancer
  1. Juraj Adamik1,
  2. Adam Grant1,
  3. Damian Trujillo1,
  4. Rakesh Kumar Goyal1,
  5. William Ho1,
  6. Michael Chisamore2,
  7. Shoji Ikeda1,
  8. Marvin Au1,
  9. Denise Nagata1,
  10. Eugene Lurie1,
  11. Qingfeng Zhu3,
  12. Robert Anders3,
  13. Dirk G Brockstedt1,
  14. Mohsen Sabouri Ghomi1 and
  15. Paul D Kassner1
  1. 1RAPT Therapeutics, Inc., South San Francisco, CA, USA
  2. 2Merck and Co., Inc., Rahway, NJ, USA
  3. 3Johns Hopkins University School of Medicine, Baltimore, MD, USA
  • Journal for ImmunoTherapy of Cancer (JITC) preprint. The copyright holder for this preprint are the authors/funders, who have granted JITC permission to display the preprint. All rights reserved. No reuse allowed without permission.


Background FLX475 is a potent and selective CCR4 antagonist, designed to block immunosuppressive regulatory T cell (Treg) migration into the tumor microenvironment (TME), which has the potential to overcome immune resistance and broaden clinical efficacy to a variety of conventional and immunotherapy-based approaches. In a recent interim clinical update from the ongoing FLX475–02 Phase 1/2 trial (NCT03674567), evidence of monotherapy and combination activity were reported. Here, we present biomarker data from patients with multiple tumor types treated with FLX475 as monotherapy and in combination with pembrolizumab. These data support the beneficial effects of FLX475 in modification of the TME and promotion of anti-cancer immunity.

Methods and Results As determined by flow cytometry, a small but significant increase in proportion of circulating Treg (CD4+CD25+CD127-/low) was observed in patients by day 8 of treatment. Immunohistochemistry (IHC) revealed that FLX475 monotherapy increased CD8/Foxp3 density ratio, increased distance between CD8+ and Foxp3+ cells, and reduced migration of Foxp3+ cells from stroma to viable tumor regions. RNAseq data derived from tumor biopsies prior to (n = 33), and after approximately 6 weeks of treatment (n = 22, paired samples) with FLX475+/-pembrolizumab were compared to published biopsy data from anti-PD-(L)1 treated patients. Transcriptomic profiles from tumor biopsies of FLX475 monotherapy treated patients exhibited significant changes in immune pathways to resemble profiles from patients with favorable clinical outcome to anti-PD-(L)1 treatment. Analysis of paired biopsies from both FLX475/pembrolizumab and anti-PD-1 regimens showed significantly increased T cell signatures. However, FLX475/pembrolizumab prevented coordinated increase of Treg cell signatures observed in the TME of patients treated with anti-PD-1 alone. Consistent with this finding, increased expression of CCR4 and its ligands CCL17 and CCL22 were observed in biopsies of patients receiving anti-PD-1 treatment but not FLX475/pembrolizumab. To identify patients more likely to benefit from FLX475/pembrolizumab therapy, baseline transcriptomic profiles were analyzed. Patients with clinical benefit (CR, PR, and stable disease ≥6 months) were found to have elevated Treg populations compared to those without clinical benefit (PD and SD <6 months). This phenomenon was not observed in external anti-PD-1 datasets.

Conclusions FLX475 monotherapy and in combination with pembrolizumab result in beneficial changes in the TME. FLX475 monotherapy appears to modify the TME toward a phenotype associated with response to anti-PD-(L)1. Baseline markers associated with favorable outcome are different for the combination treatment compared to anti-PD-1 monotherapies suggesting that more patients might benefit from FLX475/pembrolizumab combination than pembrolizumab alone.

Ethics Approval All patients provided informed consent prior to inclusion in the study, and the protocol was approved by local institutional review boards for each clinical site. IRB Name/Approval no. or ID: Advarra/20132; BSD/IRB18-1199, Columbia Research HRPO/IRB-AAAS7290; JHM/IRB00188614/CIR00077638; Mary Crowley IRB/19-06; Medical School IRB/HUM00160818; Office of Human Subject Protection/2019-0139; Office for Human Research Studies/19-282; Univ. of Louisville IRB/19.0698; UCKA HRPP/IRB#18-001513; WCG®/20181802; Asan Medical Center IRB/S2019-0624-0001; Austin Health HREC/52407/Austin-2019; Belberry HREC/2018-08-671; CREC/CPA-CREC 044/2019; CMMC IRB/10806-002; CBNUH IRB/2019-03-010-001; Chulalongkorn Univ. IRB/1148/2019;Inje Univ. BPIRB/2021-05-020; IRB of the Univ. of Hong Kong/Hosp. Authority HK West Cluster/UW 19-394, UW 21-299; Joint Chinese Univ. of Hong Kong CREC/2019.342-T; NCKUH IRB/AB-CR-108-021; NTUH EC/201905048MSB; SMC IRB/2021-04-156-001; SNU Bundang Hosp. IRB/B-2108/703-401; SNUH IRB/H-1903-158-1023; Ulsan Univ. Hosp. IRB/UUH 2019-05-012, Yonsei Univ./4-2019-0520 and Severance Hosp. IRB/4-2019-0520.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.