You are here

Article Text

Article menu

Download PDFPDF

Poster Presentation
Immuno-imaging and -therapy in ovarian cancer and sarcoma with de novo single-chain fv-fc fusion protein targeting TEM1/CD248
  1. Chunsheng Li1,
  2. Junying Wang1,
  3. Efthymia Iliana Matthaiou1,
  4. Yi Guo1,
  5. Jia Hu1,
  6. Ann Marie Chacko2,
  7. Vladimir R Muzykantov2 and
  8. George Coukos1,3
  1. Aff1 grid.25879.310000000419368972Ovarian Cancer Research CenterUniversity of Pennsylvania Philadelphia PA USA
  2. Aff2 grid.25879.310000000419368972Institute for Translational Medicine and TherapeuticsUniversity of Pennsylvania Philadelphia PA USA
  3. Aff3 grid.9851.50000000121654204Ludwig Cancer Research CenterUniversity of Lausanne Lausanne Switzerland

https://doi.org/10.1186/2051-1426-2-S3-P219

Statistics from Altmetric.com

    Request Permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.

    Meeting abstracts

    Tumor Endothelial Marker 1 (TEM1 or CD248) has been identified as a microvascular marker of tumor angiogenesis in various human cancers. By comparing the vasculature of ovarian cancer and normal ovary, we and others [1] identified that TEM1 is overexpressed in tumor microenvironment in ovarian cancer and it could be suggested as a suitable marker for targeting tumor vasculature of cancer therapeutics. In addition, we also confirmed that TEM1 is overexpressed in cancer cells in most soft tissue sarcoma patients.

    To better assist TEM1-specific theranostics, we sought to develop TEM1-specific affinity agents. From previously isolated TEM1-specific single chain antibody, scFv78 [2], we developed a panel of new multivalent variants of scFv78 to improve the thermal, serum stability and avidity. The variants includes a dimeric Fc fusion (from hu IgG1), a tetrameric CH2 fusion, a dimeric CH3 fusion and a dimeric (Fab)2 fusion. This panel was produced using mammalian cells and affinity purified from culture supernatant. The protein of scFv78-Fc exhibited higher affinity (KD = 0.14 ± 0.01nM, 15 fold higher of parental scFv78) to the target protein in live cell ELISA assay. Using the preclinical tumor vascular models we developed [3, 4], pharmacokinetics and biodistribution studies in revealed that the scFv78Fc has extended serum half-life. Near infrared (NIR) immuno-imaging [5] and 124I-immunoPET imaging with scFv78-Fc demonstrated that such tracers were enriched in TEM1-positive tumors but not normal organs. In addition, scFv78Fc was conjugated to Monomethyl auristatin E (MMAE) and saporin to develop potent TEM1-targeting immunotoxins, which showed TEM1-specific toxicity in vitro and in preclinical animal models. Lastly, TEM1-targeted nanomedicine platform was developed and studied in vitro [6] and in several preclinical tumor models. scFv78-Fc targeted, shikonin-loaded PLGA nanoparticles showed higher accumulation and killing efficacy at the TEM1 positive tumors, compared to free drug and unarmed nanoparticle controls.

    Taken together, our data indicate that the scFv78-Fc is suitable for development as targeted therapeutics and imaging for cancers such as ovarian cancer and sarcomas. Based upon these findings, we propose to use the immuno-imaging for detection and selection of patients suitable for immune therapies with the TEM1-targeted immunotoxin or nanoparticles; and for monitoring the efficacy of such therapies.

    References

    1. 1.↵
      1. Conejo-Garcia JR,
      2. Buckanovich RJ,
      3. Benencia F, et al
      . Vascular leukocytes contribute to tumor vascularization. Blood. 2005;105:679–81. doi:10.1182/blood-2004-05-1906
      OpenUrlAbstract/FREE Full Text
    2. 2.↵
      1. Zhao A,
      2. Nunez-Cruz S,
      3. Li C, et al
      . Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. J Immunol Methods. 2011;363:221–32. pmcid:3003766 doi:10.1016/j.jim.2010.09.001
      OpenUrlCrossRefPubMed
    3. 3.↵
      1. Chacko AM,
      2. Li C,
      3. Nayak M, et al
      . Development of 124I Immuno-PET Targeting Tumor Vascular TEM1/Endosialin. Journal of nuclear medicine : official publication. 2014;500–7.
    4. 4.↵
      1. Li C,
      2. Chacko AM,
      3. Hu J, et al
      . Antibody-based tumor vascular theranostics targeting endosialin/TEM1 in a new mouse tumor vascular model. Cancer biology & therapy. 2014;15
    5. 5.↵
      1. Li C,
      2. Wang J,
      3. Hu J, et al
      . Development, Optimization, and Validation of Novel anti-TEM1/CD248 Affinity Agent for Optical Imaging in Cancer. Oncotarget. 2014;
    6. 6.↵
      1. Matthaiou E,
      2. Barar J,
      3. Sandaltzopoulos R, et al
      . Shikonin-loaded antibody-armed nanoparticles for targeted therapy of ovarian cancer. International Journal of Nanomedicine. 2014;9