Background

Immunohistochemical (IHC) detection of multiple antigens on the same tissue section represents a major unmet technological need in research and clinical diagnostics. While primary antibodies from different species have been used with differently labeled species-specific secondary antibodies, quite often the appropriate combination of antibodies is not available. More recently, primary antibodies from the same species have been employed for multiplex IHC with microwaving treatment between each antigen staining cycle. This manual microwaving method prevents the cross-reactivity of an anti-species antibody in a subsequent cycle from binding to a primary antibody at the previous cycle.