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Increased PD-L1 expression in KRAS mutated premalignant human bronchial epithelial cells is enhanced by LKB1 loss and mediated by ERK activation
  1. Mi-Heon Lee1,
  2. Jane Yanagawa1,
  3. Rui Li1,
  4. Tonya C Walser1,
  5. Kostyantyn Krysan1,
  6. Gerald Wang1,
  7. Jonathan W Goldman1,
  8. Edward B Garon Garon1,
  9. Gang Zeng1,
  10. Sherven Sharma1,
  11. John D Minna2,
  12. David Carbone3,
  13. Steven M Dubinett1 and
  14. Jay M Lee1
  1. Aff1 grid.19006.3e0000000096326718UCLA Los Angeles CA USA
  2. Aff2 grid.267313.20000000094827121UTSW Dallas TX USA
  3. Aff3 grid.261331.40000000122857943The Ohio State University Columbus OH USA

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Meeting abstracts


PD-1/PD-L1 immune checkpoint pathway mediates tumor evasion from the immune system, and may be associated with poor prognosis in lung cancer. Activating KRAS mutations and LKB1 loss are common mutations in non-small cell lung carcinoma (NSCLC). Patients with mutated KRAS demonstrate less benefit from chemotherapy and resistance to approved targeted therapies. Inactivation of tumor suppressors commonly co-exist with KRAS mutations, and may contribute to tumor progression and treatment response. LKB1 is a tumor suppressor gene commonly inactivated in lung adenocarcinomas. The role of LKB1loss as a prognostic or predictive marker within human KRAS mutant NSCLC is unclear. There have been no therapeutic strategies that effectively target LKB1. Furthermore, the effect of these mutations on immune checkpoint immune regulation is poorly understood. KRAS mutations are known to activate the RAF-MEK-ERK pathway. We hypothesize that KRAS mutations directly regulate the PD-1/PD-L1 pathway and LKB1 loss may increase this effect through ERK activation.


Immortalized human bronchial epithelial cells (HBEC) with null vector and shRNA control (HBEC-vector-sh control), HBEC LKB1 knockdown (HBEC-vector-shLKB1), KRAS–mutated (KRASv12) HBEC cells with shRNA control (HBEC-KRAS-sh control), and LKB1 knockdown KRAS–mutated HBEC cells (HBEC-KRAS-shLKB1) were used to assess mRNA and/or surface protein expression levels of PD-L1 by real time-qPCR (RT-qPCR) and flow cytometry. HBEC-vector and HBEC-KRAS cells were treated with MEK (ERK kinase) inhibitor (PD0325901) at 1uM for 24hrs and evaluated for mRNA and surface protein expression of PD-L1.


PD-L1 mRNA level increased 2.4 fold (HBEC-KRAS-sh control; p < 0.05) and 4 fold (HBEC-KRAS-shLKB1; p < 0.05) vs. HBEC-vector-sh control. Based on mean fluorescence intensity, PD-L1 protein expression was 7.4 (HBEC-KRAS-sh control) and 12.1 (HBEC-KRAS-shLKB1) vs. 3.4 (HBEC-vector-sh control). With ERK inhibition, PD-L1 mRNA levels decreased 88% (HBEC-KRAS-sh control) and 68% (HBEC-KRAS-shLKB1), and PD-L1 surface protein levels were reduced 77% (HBEC-KRAS-sh control) and 64% (HBEC-KRAS-shLKB1). These findings suggest that ERK activation mediates KRAS mutation driven over-expression of PD-L1 mRNA and protein, which is further increased with LKB1 loss. LKB1 loss alone without KRAS mutation did not significantly affect PD-L1 expression (HBEC-vector-shLKB1 vs. HBEC-vector-sh control).


Here, we demonstrate that elevated PD-L1 expression in premalignant KRAS mutated human bronchial epithelial cells is enhanced by LKB1 loss and mediated by MAPK/ERK pathway activation. Our findings suggest that activating KRAS mutations and LKB1 loss in NSCLC may predict improved therapeutic efficacy to PD-1/PD-L1 inhibition.