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  • Preliminary analysis of immune responses in patients enrolled in a Phase II trial of cyclophosphamide with allogenic dribble vaccine alone (DPV-001) or with GM-CSF or imiquimod for adjuvant treatment of stage IIIa or IIIb NSCLC

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Preliminary analysis of immune responses in patients enrolled in a Phase II trial of cyclophosphamide with allogenic dribble vaccine alone (DPV-001) or with GM-CSF or imiquimod for adjuvant treatment of stage IIIa or IIIb NSCLC
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  1. Rachel Sanborn1,
  2. Brian Boulmay2,
  3. Rui Li3,
  4. Bradley Spieler2,
  5. Kyle Happel2,
  6. Christopher Paustian4,
  7. Tarsem Mougdil1,
  8. Zipei Feng5,
  9. Christopher Dubay1,
  10. Brenda Fisher1,
  11. Yoshinobu Koguchi5,
  12. Sandra Aung6,
  13. Eileen Mederos2,
  14. Carlo Bifulco4,
  15. Michael McNamara4,
  16. Keith S Bahjat1,
  17. William Redmond4,
  18. Augusto C Ochoa2,
  19. Hong Ming Hu1,
  20. Bernard Fox5,
  21. Walter Urba5 and
  22. Traci Hilton7
  1. Aff1 grid.415337.7Robert W. Franz Cancer Research Center, Earle A. Chiles Research InstituteProvidence Cancer Center Portland OR USA
  2. Aff2 grid.279863.10000000089541233Stanley S. Scott Cancer Center, School of MedicineLSUHSC, New Orleans, LA New Orleans LA USA
  3. Aff3 grid.415337.7Providence Cancer Center Portland OR USA
  4. Aff4 grid.415337.7Earle A. Chiles Research InstituteProvidence Cancer Center Portland OR USA
  5. Aff5 grid.415286.c0000 0004 0463 5556Earle A. Chiles Research Institute Portland OR USA
  6. Aff6 grid.437284.ePrometheus Laboratories Inc. San Diego CA USA
  7. Aff7 grid.438792.3UbiVac Portland OR USA

https://doi.org/10.1186/2051-1426-3-S2-P435

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    Meeting abstracts

    Background

    Tumor-derived autophagosomes, DRibbles, are DC-targeted microvesicles containing more than 100 putative NSCLC antigens, many as potential altered-peptide ligands (APL), which could increase their immunogenicity. The microvesicles also contain at least 15 DAMPs with agonist activity for TLR 2, 3, 4, 7 and 9. In preclinical models DRibble immunotherapy provided significant cross-protection against 8 of 9 tumors and was effective in treating established tumors. We hypothesize that the efficacy of DRibbles' vaccination stems from their ability to present stabilized tumor-derived short-lived proteins (SLiPs) and defective ribosomal products (DRiPs) that are normally not processed and presented by professional antigen presenting cells (APCs). These SLiPs and DRiPs represent a potential pool of tumor antigens against which the host is not tolerant. The DPV-001 vaccine is made up of DRibbles produced from two cell lines, UbiLT3 and UbiLT6. The priming vaccination includes DRibbles from both cell lines, while subsequent administrations alternate between DRibbles derived from the two cell lines.

    Methods and results

    Patients are vaccinated at 3-week intervals, alone or with assigned adjuvant (GM-CSF or imiquimod), for a total of 7 doses, and an option to continue vaccinating at 6 week intervals (without adjuvant). PBMCs and serum are collected at baseline and at each vaccination. Immune monitoring panels are run on peripheral whole blood to evaluate lymphocyte populations and their activation status. PBMCs from the baseline visit and week 12/13 are stimulated with DRibbles (vaccine and control) to measure vaccine-specific cytokine production. Patient serum from the baseline visit and week 12/13 is analyzed for antibody response to >9000 human proteins using ProtoArrays. Whole exome sequencing of tumors and normal tissue is performed when possible to evaluate antibody responses to mutations and altered peptide ligands. Nanostring and multispectral IHC are being used to evaluate tumor immune biomarkers when tissue is available.

    Conclusions

    The DPV-001 vaccine provides a source of broad-spectrum relevant antigens. Preliminary analyses of patients receiving the DPV-001 vaccine show effects on T cells and B cells with increased antibody responses at 12 or 13 weeks.

    Clinical Trial Identifier: NCT01909752, Support: R44 CA121612-02A1.

    Trial Registration

    ClinicalTrials.gov identifier NCT01909752.