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Costimulation to enhance the antitumor activity of CD19 eng T cells
  1. Mireya Velasquez1,
  2. David Torres2,
  3. Aarohi Thakkar1,
  4. Sunitha Kakarla1,
  5. Carolyn Arber Barth1,
  6. Xiao-Tong Song2 and
  7. Stephen Gottschalk1
  1. Aff1 grid.39382.33000000012160926XBaylor College of Medicine, Center for Cell and Gene Therapy Houston TX USA
  2. Aff2 grid.39382.33000000012160926XBaylor College of Medicine Houston TX USA

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Meeting abstracts


Immunotherapy with T cells or bispecific T cell engagers is one promising approach to improve outcomes for patients with CD19-positive hematological malignancies. We had previously shown that T cells expressing bispecific T cell engagers that recognize CD19 and CD3 (CD19-ENG T cells) are activated in an antigen dependent manner, recruit resident T cells to tumors, and have anti-tumor activity in preclinical models. In this project we now wanted to evaluate if provision of co-stimulation enhances the effector function of CD19-ENG T cells by expressing CD80 and 41BBL on their cell surface (CD19 ENG/costim T cells).


CD19-ENG T cells were generated by transducing T cells with a retroviral vector encoding a CD19-specific T cell engager, and CD19-ENG/Costim T cells were generated by double transducing T cells with the previous construct and a 2nd retroviral vector encoding the costimulatory molecules 41BBL and CD80. The effector function of the generated T cells was evaluated in vitro and in a xenograft model.


CD19-ENG and CD19-ENG/Costim T cells recognized CD19+ lymphoma (Daudi, Raji) and acute leukemia (BV173) cells as judged by IFN-g secretion. Both ENG T cell populations produced IL-2 in the presence of CD19-positive targets expressing CD80 and CD86 (Daudi and Raji). However, CD19-ENG/Costim T cells produced higher levels of IL-2 in comparison to CD19-ENG T cells after stimulation with BV173 (CD19+CD80-CD86-). ENG and ENG/Costim T cells specific for an irrelevant antigen (EphA2) did not produce cytokines, confirming antigen dependence. Specificity was confirmed in cytotoxicity assays. In vivo anti-tumor activity of CD19-ENGand CD19-ENG/Costim T cells was assessed in a BV173/NSG xenograft model. First we determined the minimal required CD19-ENG T cell dose to observe anti-tumor effects. Three injections of 1x107 CD19-ENG T cells cured 5/5 mice, one injection 2/5 mice, and one dose of 1x106 CD19-ENG T cells had no anti-tumor effects. In contrast one dose of 1x106 or 1x107 CD19-ENG/Costim T cells cured 5/5 mice. ENG T cells or ENG/Costim T cells recognizing EphA2 had no anti-tumor effects.


Provision of co-stimulation increases the effector function of CD19-ENG T cells resulting in enhanced anti-tumor activity in vivo. Genetically modifying T cells to express engagers and additional molecules to enhance their effector function may present a promising alternative to current CD19-targeted immunotherapies.