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Poster Presentation
Immune biomarkers associated with clinical benefit from atezolizumab (MPDL3280a; anti-PD-L1) in advanced urothelial bladder cancer (UBC)
  1. Thomas Powles1,
  2. Dorothee Nickles2,
  3. Eliezer Van Allen3,
  4. Colombe Chappey2,
  5. Wei Zou2,
  6. Marcin Kowanetz2,
  7. Edward Kadel2,
  8. Mitchell Denker2,
  9. Zachary Boyd2,
  10. Nicholas Vogelzang4,
  11. Joseph Kim5,
  12. Joaquim Bellmunt3,
  13. Yohann Loriot6,
  14. Charles G Drake7,
  15. Carol O'Hear2,
  16. Marcella Fasso2,
  17. Priti Hegde2 and
  18. Sanjeev Mariathasan2
  1. Aff1 grid.4868.20000000121711133Barts Cancer InstituteQueen Mary University of London London UK
  2. Aff2 grid.418158.10000000405344718Genentech, Inc. South San Francisco CA USA
  3. Aff3 grid.65499.370000000121069910Dana-Farber Cancer Institute Boston MA USA
  4. Aff4 grid.428254.dUS Oncology ResearchComprehensive Cancer Centers Las Vegas NV USA
  5. Aff5 grid.433818.5Yale Cancer Center New Haven CT USA
  6. Aff6 grid.5842.b0000000121712558Gustav Roussy Institute of OncologyParis-Sud University Paris France
  7. Aff7 grid.21107.350000000121719311Department of OncologySidney Kimmel Comprehensive Cancer Center at Johns Hopkins University Baltimore MD USA

https://doi.org/10.1186/2051-1426-3-S2-P83

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    Meeting abstracts

    Background

    Atezolizumab (anti-PD-L1) has demonstrated robust clinical activity in UBC [1]. Elevated PD-L1 expression on tumor-infiltrating immune cells (IC) is associated with increased clinical efficacy; however, the contribution of other immune biomarkers is unknown. In this study, we explored tumor-based and circulating biomarkers and their correlation with clinical benefit in atezolizumab-treated patients with UBC.

    Methods

    Patients from the UBC cohort (n = 92) of the Phase Ia atezolizumab trial PCD4989g (NCT01375842) served as source population for tumor specimens, plasma and PBMC. Baseline tumor PD-L1 expression was assessed by immunohistochemistry using the SP142 antibody assay optimized to detect PD-L1 on both tumor cells (TC) and IC. RNA gene expression on tumor and PBMC samples was interrogated with a NanoString panel of 800 immune and cancer genes. Sequential blood draws assessed dynamic changes in circulating immune biomarkers in plasma (RBM, Multi-Analyte Platform). Correlation between biomarker expression and 6-month progression-free survival (PFS; as a measure of clinical benefit) was assessed.

    Results

    Baseline gene expression in tumors revealed an effector T cell signature (including CD8A, GZMA, IFNG) and NK gene signature (NKG2 family members) associated with clinical benefit. In contrast, disease progression was associated with either a concomitant presence of the immune signature and an opposing stromal signature (PDPN, COL5A1, etc) or the absence of both signatures. Expression of T cell effector and immune checkpoint genes (CTLA4, PD-1, TIGIT, LAG3) correlated with PD-L1 expression on IC but not TC. Increased expression of myeloid-derived cytokines (IL-6 and IL-8) in the plasma was associated with lack of clinical benefit. Moreover, on-treatment sampling revealed an increased plasma HCG, CA15-3 and TIMP-1 to be correlated with disease progression. Immune biomarkers associated with PBMC, as well as tumor biomarkers associated with various tumor subtypes, will also be discussed.

    Conclusions

    Our findings indicate that clinical benefit (as defined by 6-month PFS) from atezolizumab is influenced by a pre-existing CD8+ effector T cell and NK cytolytic gene signature in the tumor, which correlated with IC PD-L1 expression. Increased stromal and myeloid-derived cytokine expression in tumor and plasma, respectively, were associated with lack of clinical benefit, underscoring the complex interplay among immunological components in UBC. These components may be conceivable targets to overcome potential resistance and promote response to atezolizumab.

    ClinicalTrials.gov Identifier: NCT01375842

    References

    1. 1.↵
      1. Powles T,
      2. Eder JP,
      3. Fine GD,
      4. Braiteh FS,
      5. Loriot Y,
      6. Cruz C, et al
      . MPDL3280A (anti-PD-L1) treatment leads to clinical activity in metastatic bladder cancer. Nature. 2014;515:7528558–562. doi:10.1038/nature13904
      OpenUrlCrossRefPubMedWeb of Science