Article Text

Download PDFPDF

P06.02 Cancer-specific differences of tertiary lymphoid structures and cellular responses against frequently expressed cancer testis antigens
  1. E Preugszat1,
  2. M Thelen1,
  3. M Garcia-Marquez1,
  4. J Lehmann1,
  5. D Keller1,
  6. M von Bergwelt-Baildon1,2,3 and
  7. HA Schlößer1,4
  1. 1Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany
  2. 2Department of Internal Medicine III, University Hospital, Ludwig Maximilian University, Munich, Germany
  3. 3German Cancer Consortium (DKTK), Heidelberg, Heidelberg, Germany
  4. 4Department of General, Visceral, Cancer and Transplantation Surgery, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany


Background Tertiary lymphocyte structures (TLS) can be detected in the tumor microenvironment across a wide range of cancer types and are associated with increased patient survival and susceptibility to immune checkpoint inhibition. However, evidence for the functional significance of TLS on humoral and cellular immunity is scarce. In this study, we combine assessment of abundance and spatial distribution of TLS with expression levels of 10 tumor associated antigens (TAAs) and functional analyses of T cell responses to these antigens.

Materials and Methods 52 treatment naïve cancer patients across 5 tumor types (NSCLC, CRC, RCC, HCC and BCA) were included. Presence and localization of TLS was assessed in immunohistochemical stainings (CD20) of whole section slides from FFPE embedded tumor samples. B cell clusters were quantified in the whole tumor region and in two different tumor margins (300 µm, 2000 µm). A panel of 30 cancer testis antigens was selected via GEPIA software (TCGA Database) and their expression in our cohort was determined using NanoString based RNA expression analysis of tumor samples and patient-matched healthy tissue. The 10 peptide pools with the largest cross-cancer overlap were selected based on our NanoString results. 2-color Fluorospot assays (IFN-γ and IL-2) were applied to assess the frequency of tumor-specific T-cell responses in patient PBMCs (triplicates for each TAA).

Results CD20 immunohistochemistry and enumeration of intra- and peritumoral TLS revealed different distribution patterns of TLS/mm2 with the largest proportion in the 300 µm margin (p < 0.01) in most of the cancer types. This effect was particularly observed in patients with non-small cell lung cancer (NSCLC). The 10 tumor antigens CEP55, CT83, GAGE1, IGF2BP3, MAGEA1, MAGEA3/6, PBK, PRAME, Survivin and TTK were selected as they showed the highest overlap across different cancer types and the most pronounced differential expression between tumor and matched normal tissue. While 31/52 (59.6%) patients showed an IFN-γ, only 11/52 (21.2%) patients exhibited an IL-2 response against at least one of the tested CTAs. Survivin was the CTA presenting the highest frequency of responses (18/52 IFN-γ and 5/52 IL-2 responses). PBMCs of patients with NSCLC showed the highest frequency of T-cell responses (83.3% with at least one IFN-γ response) and patients with HCC and BCA the lowest proportion (40.0%).

Conclusions The observed findings underline the importance of TLS as a novel biomarker and a possible association to cellular responses may even enhance their prognostic value. Our planned analyses of combined humoral immune responses will further elucidate the role of TLS in anti-tumor immune response of the analyzed cancer types. A combined targeting of a predefined or personalized set of included TAAs appears promising across the different cancer types.

Disclosure Information E. Preugszat: B. Research Grant (principal investigator, collaborator or consultant and pending grants as well as grants already received); Modest; Koeln Fortune Program, Faculty of Medicine, University of Cologne. M. Thelen: None. M. Garcia-Marquez: None. J. Lehmann: None. D. Keller: None. M. von Bergwelt-Baildon: Other; Modest; Honoraria for advisory boards, for invited talks from BMS and financial support for research projects from Astellas, Roche and MSD. H.A. Schlößer: Other; Significant; Financial support for research projects from Astra Zeneca.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.