Article Text

Download PDFPDF

83 Spatially resolved transcriptomic and proteomic investigation of breast cancer and its immune microenvironment
  1. Jennifer Chew,
  2. Cedric Uytingco1,
  3. Rapolas Spalinskas1,
  4. Yifeng Yin1,
  5. Joe Shuga1,
  6. Benton Veire1,
  7. Naishitha Anaparthy1,
  8. Ryo Hatori1,
  9. Anna-Maria Katsor1,
  10. Layla Katiraee1,
  11. Alexander Hermes1,
  12. Jun Ding Chiang1,
  13. Patrick Roelli1,
  14. Stephen Williams1,
  15. William Nitsch1,
  16. Neil Weisenfeld1,
  17. Dan Walkser1,
  18. Jason Koth1,
  19. Subham Basu2,
  20. Will Howat2,
  21. Karthik Ganapathy1 and
  22. Marlon Stoeckius1
  1. 110X Genomics, Pleasanton, CA, USA
  2. 2Abcam, Cambridge, UK


Background The tumor microenvironment (TME) is composed of highly heterogeneous extracellular structures and cell types such as endothelial cells, immune cells, and fibroblasts that dynamically influence and communicate with each other. The constant interaction between a tumor and its microenvironment plays a critical role in cancer development and progression and can significantly affect a tumor’s response to therapy and capacity for multi-drug resistance. High resolution analyses of gene and protein expression with spatial context can provide deeper insights into the interactions between tumor cells and surrounding cells within the TME, where a better understanding of the underlying biology can improve treatment efficacy and patient outcomes. Here, we demonstrated the ability to perform streamlined multi-omic tumor analyses by utilizing the 10X Genomics Visium Spatial Gene Expression Solution for FFPE with multiplex protein enablement. This technique simultaneously assesses gene and protein expression to elucidate the immunological profile and microenvironment of different breast cancer samples in conjunction with standard pathological methods.

Methods Serial (5 µm) sections of FFPE human breast cancer samples were placed on Visium Gene Expression (GEX) slides. The Visium GEX slides incorporate ~5,000 molecularly barcoded, spatially encoded capture spots onto which tissue sections are placed, stained, and imaged. Following incubation with a human whole transcriptome, probe-based RNA panel and an immuno-oncology oligo-tagged antibody panel, developed with Abcam conjugated antibodies, the tissues are permeabilized and the representative probes are captured. Paired GEX and protein libraries are generated for each section and then sequenced on an Illumina NovaSeq at a depth of ~50,000 reads per spot. Resulting reads from both libraries are aligned and overlaid with H&E-stained tissue images, enabling analysis of both mRNA and protein expression. Additional analyses and data visualizations were performed on the Loupe Browser v4.1 desktop software.

Conclusions Spatial transcriptomics technology complements pathological examination by combining histological assessment with the throughput and deep biological insight of highly-multiplexed protein detection and RNA-seq. Taken together, our work demonstrated that Visium Spatial technology provides a spatially-resolved, multi-analyte view of the tumor microenvironment, where a greater understanding of cellular behavior in and around tumors can help drive discovery of new biomarkers and therapeutic targets.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.