PT  - JOURNAL ARTICLE
AU  - Xiong, Ying
AU  - Wang, Zewei
AU  - Zhou, Quan
AU  - Zeng, Han
AU  - Zhang, Hongyu
AU  - Liu, Zhaopei
AU  - Huang, Qiuren
AU  - Wang, Jiajun
AU  - Chang, Yuan
AU  - Xia, Yu
AU  - Wang, Yiwei
AU  - Liu, Li
AU  - Zhu, Yu
AU  - Xu, Le
AU  - Dai, Bo
AU  - Bai, Qi
AU  - Guo, Jianming
AU  - Xu, Jiejie
TI  - Identification and validation of dichotomous immune subtypes based on intratumoral immune cells infiltration in clear cell renal cell carcinoma patients
AID  - 10.1136/jitc-2019-000447
DP  - 2020 Mar 01
TA  - Journal for ImmunoTherapy of Cancer
PG  - e000447
VI  - 8
IP  - 1
4099  - http://jitc.bmj.com/content/8/1/e000447.short
4100  - http://jitc.bmj.com/content/8/1/e000447.full
SO  - J Immunother Cancer2020 Mar 01; 8
AB  - Background Increasing evidence has elucidated the clinical significance of tumor infiltrating immune cells in predicting outcomes and therapeutic efficacy. In this study, we comprehensively analyze the tumor microenvironment (TME) immune cell infiltrations in clear cell renal cell carcinoma (ccRCC) and correlated the infiltration patterns with anti-tumor immunity and clinical outcomes.Methods We analyzed immune cell infiltrations in four independent cohorts, including the KIRC cohort of 533 patients, the Zhongshan ccRCC cohorts of 259 patients, the Zhongshan fresh tumor sample cohorts of 20 patients and the Zhongshan metastatic ccRCC cohorts of 87 patients. Intrinsic patterns of immune cell infiltrations were evaluated for associations with clinicopathological characteristics, underlying biological pathways, genetic changes, oncological outcomes and treatment responses.Results Unsupervised clustering of tumor infiltrating immune cells identified two microenvironment subtypes, TMEcluster-A and TMEcluster-B. Gene markers and biological pathways referring to immune evasion were upregulated in TMEcluster-B. TMEcluster-B associated with poor overall survival (p<0.001; HR 2.629) and recurrence free survival (p=0.012; HR 1.870) in ccRCC validation cohort. TMEcluster-B cases had worse treatment response (p=0.009), overall survival (p<0.001; HR 2.223) and progression free survival (p=0.015; HR 2.7762) in metastatic ccRCC cohort. The predictive accuracy of International Metastatic Database Consortium risk score was improved after incorporation of TME clusters.Conclusions TMEcluster-A featured increased mast cells infiltration, prolonged survival and better treatment response. TMEcluster-B was a heavily infiltrated but immunosuppressed phenotype enriched for macrophages, CD4+ T cells, Tregs, CD8+ T cells and B cells. TMEcluster-B predicted dismal survival and worse treatment response in clear cell renal cell carcinoma patients.