PT  - JOURNAL ARTICLE
AU  - Aiken, Taylor J
AU  - Komjathy, David
AU  - Rodriguez, Matthew
AU  - Stuckwisch, Ashley
AU  - Feils, Arika
AU  - Subbotin, Vladimir
AU  - Birstler, Jen
AU  - Gillies, Stephen D
AU  - Rakhmilevich, Alexander L
AU  - Erbe, Amy K
AU  - Sondel, Paul M
TI  - Short-course neoadjuvant in situ vaccination for murine melanoma
AID  - 10.1136/jitc-2021-003586
DP  - 2022 Jan 01
TA  - Journal for ImmunoTherapy of Cancer
PG  - e003586
VI  - 10
IP  - 1
4099  - http://jitc.bmj.com/content/10/1/e003586.short
4100  - http://jitc.bmj.com/content/10/1/e003586.full
SO  - J Immunother Cancer2022 Jan 01; 10
AB  - Background Surgical resection remains an important component of multimodality treatment for most solid tumors. Neoadjuvant immunotherapy has several potential advantages, including in-situ tumor vaccination and pathologic assessment of response in the surgical specimen. We previously described an in-situ tumor vaccination strategy in melanoma using local radiation (RT) and an intratumoral injection of tumor-specific anti-GD2 immunocytokine (IT-IC). Here we tested whether neoadjuvant in-situ tumor vaccination using anti-GD2 immunocytokine and surgical resection, without RT, could generate immunologic memory capable of preventing recurrence or distant metastasis.Methods Mice bearing GD2 expressing B78 melanoma tumors were treated with neoadjuvant radiation, IT-IC, or combined RT + IT-IC. Surgical resection was performed following neoadjuvant immunotherapy. Immune infiltrate was assessed in the resection specimens. Mice were rechallenged with either B78 contralateral flank tumors or pulmonary seeding of non-GD2 expressing B16 melanoma metastasis induced experimentally. Rejection of rechallenge in mice treated with the various treatment regimens was considered evidence of immunologic memory.Results Neoadjuvant IT-IC and surgical resection resulted in increased CD8 T cell infiltration, a higher CD8:regulatory T cell ratio, and immunologic memory against contralateral flank rechallenge. The timing of resection did not significantly impact the development of memory, which was present as early as the day of surgery. There was less local wound toxicity with neoadjuvant IT-IC compared with neoadjuvant RT +IT IC. Neoadjuvant IT-IC and resection resulted in the rejection of B16 lung metastasis in a CD4 T cell dependent manner.Conclusions Neoadjuvant IT-IC generates immunologic memory capable of preventing distant metastasis despite limited efficacy against large primary melanoma tumors. By combining neoadjuvant tumor vaccination and surgery, the toxicity of local RT was avoided. These preclinical data support further investigation regarding the use of neoadjuvant IT-IC in patients with melanoma at high risk for occult distant disease.Data are available upon reasonable request. The data sets generated and/or analyzed during the current study are not publicly available due to their relevance only for the experiments presented here but are available from the corresponding author on reasonable request.