PT - JOURNAL ARTICLE AU - DeVorkin, Lindsay AU - Mai, Juntao (Matt) AU - Caldwell, Kate AU - Jacobs, Tim AU - Tonikian, Raffi AU - Herve, Karine AU - Hwang, Yuri AU - Faralla, Cristina AU - Wei, Wei AU - Lathouwers, Emma AU - Chappell, Rhys AU - Hannie, Stefan AU - Lam, Katherine AU - Dhupar, Harveer AU - Tran, Tran AU - Cid, Melissa AU - Bolten, Lena AU - Pinsky, Tova AU - Xiang, Ping AU - Lai, Courtenay AU - Lee, Ahn AU - Chan, Patrick AU - Chin, Jasmine AU - Yamniuk, Aaron AU - Dalal, Kush AU - Barnhart, Bryan TI - 1196 Streamlining T cell engager development with a diverse panel of fully human CD3-binding antibodies, bispecific engineering technology, and an integrated discovery engine AID - 10.1136/jitc-2022-SITC2022.1196 DP - 2022 Nov 01 TA - Journal for ImmunoTherapy of Cancer PG - A1240--A1240 VI - 10 IP - Suppl 2 4099 - http://jitc.bmj.com/content/10/Suppl_2/A1240.short 4100 - http://jitc.bmj.com/content/10/Suppl_2/A1240.full SO - J Immunother Cancer2022 Nov 01; 10 AB - Background CD3 T cell engagers have the potential to be a cornerstone of immuno-oncology. However, a limited pool of CD3-binding antibodies and technological challenges in engineering bispecifics have hindered development. Discovering effective T cell engagers requires two target-binding arms — a CD3 arm that fine-tunes T cell activation and a tumor arm with high specificity for cancer cells — optimized as a whole to work in concert with each other. Beginning with diverse panels of antibodies increases the probability of finding appropriately potent and developable T cell engagers and reduces the need for downstream engineering.Methods We used microfluidic technology to screen more than 3.5 million single cells from humanized mice and identified >200 CD3-specific antibodies. Using high-throughput assays, we determined affinity for CD3εδ and CD3εγ, cross-reactivity to human and cyno primary T cells, CD3 binding kinetics, and epitope bins. We assessed T cell activation by measuring CD25 and CD69 expression by flow cytometry. We then used our bispecific engineering platform, OrthoMabTM, to generate a proof-of-concept panel of CD3 x EGFR bispecific antibodies. Developability properties were assessed, including hydrophobicity (aHIC), self-association (AC-SINS), polyspecificity (BVP-ELISA), stability (nanoDSF), and aggregation (aSEC). CD3 T cell engager potencies were measured using an NFAT reporter T cell activation assay and an xCELLigence tumor cell killing assay, and cytokine release was assessed by FLEXMAP CD.Results We identified hundreds of fully human CD3-specific antibodies that are diverse, developable, and validated. The antibodies displayed a wide range of CD3 binding affinities (KD ~1 nM to 1 μM), binding kinetics, and T cell activation potencies (EC50 ~6 to 190 nM). Data on this novel panel includes epitope binning, which revealed human and cyno CD3-binders that are distinct from previously described cross-reactive antibodies. The antibodies were assessed using a range of biophysical assays and have favorable developability properties. In an expanded proof-of-concept study, we used OrthoMabTM to generate a panel of CD3 x EGFR bispecific antibodies. The resulting bispecifics had favorable developability properties, and displayed a wide range of antigen-dependent T cell activation (EC50 ~2 pM to 2 nM) and tumor cell killing potencies (EC50 ~0.01 to 1 nM). From this panel, we identified potent T cell engagers that achieved >90% tumor cell killing with low levels of cytokine release.Conclusions By integrating our panel of CD3-binding antibodies with our bispecific engineering and high-throughput antibody assessment capabilities, we identified developable CD3 T cell engagers with potent tumor cell-killing activity and minimal cytokine release.