PT - JOURNAL ARTICLE AU - Trieu, Vuong AU - D'Cruz, Osmond TI - 599 Mechanism of action for OT-101 TGF-ß immunotherapy AID - 10.1136/jitc-2022-SITC2022.0599 DP - 2022 Nov 01 TA - Journal for ImmunoTherapy of Cancer PG - A627--A627 VI - 10 IP - Suppl 2 4099 - http://jitc.bmj.com/content/10/Suppl_2/A627.short 4100 - http://jitc.bmj.com/content/10/Suppl_2/A627.full SO - J Immunother Cancer2022 Nov 01; 10 AB - Background OT-101 is being developed as immunotherapy for a broad range of cancers. Cancers overexpress TGF-β, which suppresses host innate immune response to the cancers. Treatment with OT-101 lifts the TGF-β cloaking effect and allows innate or therapeutic immunity to attack and eliminate the cancers. OT-101 completed phase 2 for pancreatic cancer and melanoma and phase 2 in glioblastoma with robust efficacy and safety.Methods Pharmacokinetic analysis of OT-101/trabedersen for P001 study was assessed over the first two cycles of 7- or 4-day intravenous infusions, separated by 7- or 10-day treatment-free interval, respectively, at doses of 40, 80, 140, 160, 190, 240, 250, and 330 mg/m2/day to demonstrated dose exposure response. Xenograft studies were performed across immunocompetent mice (C57Bl6), humanized immune mice (SCID), and T-cell deficient immune mice (nude) to demonstrate immune cell responses.Results The median AUClast was 232 ug*h/mL (29.7-834) across the three tumor types (pancreatic cancer (PC), melanoma (Mel), and colorectal cancer (CRC)). OT-101 PK is dose proportional (p<0.0001). The PK is highly variable between patients with the AUClast of 140 mg/m2 spanning the range of observed values for the four dose levels examined for 4 days on 10 days off schedule (140, 190, 250, 330). Patients with AUC > median exhibited improved PFS for Mel and CRC but not for PC with median PFS of 67 vs. 49 days, p=0.005, 84 vs. 40 days, p=ns, and 55 vs. 56 days, p= ns, respectively. More than half of the OT-101 treated PC patients went into long term disease control (21 of 37 pts, 55%) allowing them to enter into subsequent chemotherapy which has an unexpected benefit of more than doubling their median OS, 9.3 vs. 2.6 mos, p<0.0001. Among those who underwent subsequent chemotherapy, high AUC was associated with improved OS, 9.6 vs. 2.4 mos, p=0.0006. Animal xenograft studies demonstrated robust immune cell infiltration of the tumors. Synergy demonstrated when OT-101 combined with immunotherapy or chemotherapy.Conclusions Suppression of the TGF-β resulted in conversion of cold to hot tumors with dose dependent relationship. The synergy between OT-101 chemotherapy is similar to OT-101 immunotherapy suggesting that chemotherapy is inducing immune responds amplified with prior treatment of OT-101. OT-101 is currently being combined with IL2, PD-1, and PDL-1 agents in multiple phase 2 trials.Trial Registration NCT00844064- Safety and Tolerability of AP 12009, Administered I.V. in Patients With Advanced Tumors Known to Overproduce TGF-beta-2