%0 Journal Article %A Edmund Poon %A Stefanie Mullins %A Amanda Watkins %A Geoffrey S. Williams %A Jens-Oliver Koopmann %A Gianfranco Di Genova %A Marie Cumberbatch %A Margaret Veldman-Jones %A Shaun E. Grosskurth %A Vasu Sah %A Alwin Schuller %A Corrine Reimer %A Simon J. Dovedi %A Paul D. Smith %A Ross Stewart %A Robert W. Wilkinson %T The MEK inhibitor selumetinib complements CTLA-4 blockade by reprogramming the tumor immune microenvironment %D 2017 %R 10.1186/s40425-017-0268-8 %J Journal for ImmunoTherapy of Cancer %P 63 %V 5 %N 1 %X Background T-cell checkpoint blockade and MEK inhibitor combinations are under clinical investigation. Despite progress elucidating the immuno-modulatory effects of MEK inhibitors as standalone therapies, the impact of MEK inhibition on the activity of T-cell checkpoint inhibitors remains incompletely understood. Here we sought to characterize the combined effects of MEK inhibition and anti-CTLA-4 mAb (anti-CTLA-4) therapy, examining effects on both T-cells and tumor microenvironment (TME).Methods In mice, the effects of MEK inhibition, via selumetinib, and anti-CTLA-4 on immune responses to keyhole limpet haemocyanin (KLH) immunization were monitored using ex vivo functional assays with splenocytes. In a KRAS-mutant CT26 mouse colorectal cancer model, the impact on the tumor microenvironment (TME) and the spleen were evaluated by flow cytometry. The TME was further examined by gene expression and immunohistochemical analyses. The combination and sequencing of selumetinib and anti-CTLA-4 were also evaluated in efficacy studies using the CT26 mouse syngeneic model.Results Anti-CTLA-4 enhanced the generation of KLH specific immunity following KLH immunization in vivo; selumetinib was found to reduce, but did not prevent, this enhancement of immune response by anti-CTLA-4 in vivo. In the CT26 mouse model, anti-CTLA-4 treatment led to higher expression levels of the immunosuppressive mediators, Cox-2 and Arg1 in the TME. Combination of anti-CTLA-4 with selumetinib negated this up-regulation of Cox-2 and Arg1, reduced the frequency of CD11+ Ly6G+ myeloid cells, and led to the accumulation of differentiating monocytes at the Ly6C+ MHC+ intermediate state in the tumor. We also report that MEK inhibition had limited impact on anti-CTLA-4-mediated increases in T-cell infiltration and T-cell activation in CT26 tumors. Finally, we show that pre-treatment, but not concurrent treatment, with selumetinib enhanced the anti-tumor activity of anti-CTLA-4 in the CT26 model.Conclusion These data provide evidence that MEK inhibition can lead to changes in myeloid cells and immunosuppressive factors in the tumor, thus potentially conditioning the TME to facilitate improved response to anti-CTLA-4 treatment. In summary, the use of MEK inhibitors to alter the TME as an approach to enhance the activities of immune checkpoint inhibitors warrants further investigation in clinical trials.Abbreviations:Arg1Arginase 1Cox-2Cyclooxygenase-2, also known as prostaglandin-endoperoxide synthase 2CTLA-4Cytotoxic T-lymphocyte-associated protein 4DCDendritic cellgMDSCGranulocytic myeloid-derived suppressor cellsIHCImmunohistochemicalKLHKeyhole limpet hemocyaninKRASKirsten rat sarcoma viral oncogeneLPSLipopolysaccharideMAPKMitogen-activated protein kinasesMEKMitogen-activated protein kinase kinase 1 and/or 2MHCMajor histocompatibility complexmMDSCMonocytic myeloid-derived suppressor cellsOVAOvalbuminPBMCPeripheral blood mononuclear cellsPD-1Programmed cell death protein 1pDCPlasmacytoid dendritic cellsPD-L1Programmed death-ligand 1p-ERKPhosphorylated extracellular signal-related kinasePGE2Prostaglandin E2SEAStaphylococcal enterotoxin ATAMTumor-associated macrophagesTCRT-cell receptorTh1Type 1 helperTILTumor-infiltrating lymphocyteTMETumor microenvironmentTregsRegulatory T-cellsWTWildtype %U https://jitc.bmj.com/content/jitc/5/1/63.full.pdf