TY - JOUR T1 - A systems biology approach to investigating the influence of exercise and fitness on the composition of leukocytes in peripheral blood JF - Journal for ImmunoTherapy of Cancer JO - J Immunother Cancer DO - 10.1186/s40425-017-0231-8 VL - 5 IS - 1 SP - 30 AU - Michael P. Gustafson AU - Ara Celi DiCostanzo AU - Courtney M. Wheatley AU - Chul-Ho Kim AU - Svetlana Bornschlegl AU - Dennis A. Gastineau AU - Bruce D. Johnson AU - Allan B. Dietz Y1 - 2017/12/01 UR - http://jitc.bmj.com/content/5/1/30.abstract N2 - Background Exercise immunology has become a growing field in the past 20 years, with an emphasis on understanding how different forms of exercise affect immune function. Mechanistic studies are beginning to shed light on how exercise may impair the development of cancer or be used to augment cancer treatment. The beneficial effects of exercise on the immune system may be exploited to improve patient responses to cancer immunotherapy.Methods We investigated the effects of acute exercise on the composition of peripheral blood leukocytes over time in a male population of varying fitness. Subjects performed a brief maximal intensity cycling regimen and a longer less intense cycling regimen at separate visits. Leukocytes were measured by multi-parameter flow cytometry of more than 50 immunophenotypes for each collection sample.Results We found a differential induction of leukocytosis dependent on exercise intensity and duration. Cytotoxic natural killer cells demonstrated the greatest increase (average of 5.6 fold) immediately post-maximal exercise whereas CD15+ granulocytes demonstrated the largest increase at 3 h post-maximal exercise (1.6 fold). The longer, less intense endurance exercise resulted in an attenuated leukocytosis. Induction of leukocytosis did not differ in our limited study of active (nā€‰=ā€‰10) and sedentary (nā€‰=ā€‰5) subjects to exercise although we found that in baseline samples, sedentary individuals had elevated percentages of CD45RO+ memory CD4+ T cells and elevated proportions of CD4+ T cells expressing the negative immune regulator programmed death-1 (PD-1). Finally, we identified several leukocytes whose presence correlated with obesity related fitness parameters.Conclusions Our data suggests that leukocytes subsets are differentially mobilized into the peripheral blood and dependent on the intensity and duration of exercise. Pre-existing compositional differences of leukocytes were associated with various fitness parameters.Abbreviations:BPBlood pressureDEXADual-energy X-ray absorptiometryFVCFlow-volume curvesHRHeart rateLBMLean body massMETMeasure of energy expenditure based on oxygen consumptionPETCO2Partial pressure of end-tidal carbon dioxideRERRespiratory exchange ratioRPERate of perceived exertion, Tregs, regulatory T cellsVEMinute ventilation ER -