TY - JOUR T1 - RAS status and neoadjuvant chemotherapy impact CD8+ cells and tumor HLA class I expression in liver metastatic colorectal cancer JF - Journal for ImmunoTherapy of Cancer JO - J Immunother Cancer DO - 10.1186/s40425-018-0438-3 VL - 6 IS - 1 SP - 123 AU - Fanny Ledys AU - Quentin Klopfenstein AU - Caroline Truntzer AU - Laurent Arnould AU - Julie Vincent AU - Leila Bengrine AU - Romain Remark AU - Romain Boidot AU - Sylvain Ladoire AU - Francois Ghiringhelli AU - Valentin Derangere Y1 - 2018/12/01 UR - http://jitc.bmj.com/content/6/1/123.abstract N2 - Background T lymphocytes and HLA expression on tumor cell both influence prognostic of localized colorectal cancer, but their role following chemotherapy in patients with liver metastatic colorectal cancer (mCRC) was not addressed.Methods One hundred fourteen patients treated in curative intend of liver mCRC were included in this retrospective study. Patients were either untreated or treated with neoadjuvant therapy containing an anti-EGFR, bevacizumab or oxaliplatin. Immune densities were quantified in the tumor core and in invasive margin of metastases, using Qupath software or a pathologist’s quantification. CD8, NKp46, Foxp3, CD163, HLA, PD-L1 were analyzed and were correlated with progression free survival (PFS) and overall survival (OS) using multivariable Cox proportional hazards models.Results In the whole cohort only a high CD8+ cells infiltrate, a high HLA-I expression and wild-type RAS/RAF status were associated with a better overall survival in both univariate and multivariate model. Moreover, CD8+ cells immune infiltrate at invasive margin combined to HLA expression in cancer cell could increase patient’s outcome prediction. RAS status but not immune cell infiltrate was associated with HLA expression on tumor cells. In comparison to untreated patients, neoadjuvant chemotherapy induced CD8+ cells recruitment and increased PD-L1 staining in immune infiltrates only for WT RAS patients. In this context, anti-EGFR and oxaliplatin based chemotherapy are the most powerful to induce CD8+ cells mobilization within the metastatic site.Conclusions While CD8 infiltrate and HLA expression appear to be prognostic for mCRC, CD8 and PD-L1 infiltrate are enhanced by neoadjuvant chemotherapy in mCRC under RAS status dependence.Francois Ghiringhelli and Valentin Derangere contributed equally to this work.Abbreviations:AUCArea under curveCRCColorectal cancerEGFREpidermal grow factor receptorFOLFOX5-fluorouracil, folinic acid, oxaliplatinHLAHuman leucocyte antigenINFgInterferon gammamCRCMetastatic colorectal cancerMSSMicrosatellite stabilityNANeadjuvantNKNatural killerOSOverall survivalPFSProgression free survivalTILsTumor infiltrating lymphocytesWTWild-type ER -