PT - JOURNAL ARTICLE AU - Marijo Bilusic AU - Christopher R. Heery AU - Julie M. Collins AU - Renee N. Donahue AU - Claudia Palena AU - Ravi A. Madan AU - Fatima Karzai AU - Jennifer L. Marté AU - Julius Strauss AU - Margaret E. Gatti-Mays AU - Jeffrey Schlom AU - James L. Gulley TI - Phase I trial of HuMax-IL8 (BMS-986253), an anti-IL-8 monoclonal antibody, in patients with metastatic or unresectable solid tumors AID - 10.1186/s40425-019-0706-x DP - 2019 Dec 01 TA - Journal for ImmunoTherapy of Cancer PG - 240 VI - 7 IP - 1 4099 - http://jitc.bmj.com/content/7/1/240.short 4100 - http://jitc.bmj.com/content/7/1/240.full SO - J Immunother Cancer2019 Dec 01; 7 AB - Background HuMax-IL8 (now known as BMS-986253) is a novel, fully human monoclonal antibody that inhibits interleukin-8 (IL-8), a chemokine that promotes tumor progression, immune escape, epithelial-mesenchymal transition, and recruitment of myeloid-derived suppressor cells. Studies have demonstrated that high serum IL-8 levels correlate with poor prognosis in many malignant tumors. Preclinical studies have shown that IL-8 blockade may reduce mesenchymal features in tumor cells, making them less resistant to treatment.Methods Fifteen patients with metastatic or unresectable locally advanced solid tumors were enrolled in this 3 + 3 dose-escalation trial at four dose levels (4, 8, 16, or 32 mg/kg). HuMax-IL8 was given IV every 2 weeks, and patients were followed for safety and immune monitoring at defined intervals up to 52 weeks.Results All enrolled patients (five chordoma, four colorectal, two prostate, and one each of ovarian, papillary thyroid, chondrosarcoma, and esophageal) received at least one dose of HuMax-IL8. Eight patients had received three or more prior lines of therapy and five patients had received prior immunotherapy. Treatment-related adverse events occurred in five patients (33%), mostly grade 1. Two patients receiving the 32 mg/kg dose had grade 2 fatigue, hypophosphatemia, and hypersomnia. No dose-limiting toxicities were observed, and maximum tolerated dose was not reached. Although no objective tumor responses were observed, 11 patients (73%) had stable disease with median treatment duration of 24 weeks (range, 4–54 weeks). Serum IL-8 was significantly reduced on day 3 of HuMax-IL8 treatment compared to baseline (p = 0.0004), with reductions in IL-8 seen at all dose levels.Conclusions HuMax-IL8 is safe and well-tolerated. Ongoing studies are evaluating the combination of IL-8 blockade and other immunotherapies.Trial registration NCTN, NCT02536469. Registered 23 August 2015, https://clinicaltrials.gov/ct2/show/NCT02536469?term=NCT02536469&rank=1.Marijo Bilusic and Christopher R. Heery contributed equally to this work.Abbreviations:AEsadverse eventsCIconfidence intervalCTCscirculating tumor cellsDLTdose-limiting toxicityECOGEastern Cooperative Oncology GroupEGFRepidermal growth factor receptorEMTepithelial-mesenchymal transitionIL-8Interleukin-8IL-8RIL-8 receptorirRCimmune-related response criteriaMDSCsmyeloid-derived suppressor cellsMTDmaximum tolerated doseNKnatural killerPBMCsperipheral blood mononuclear cellsPD-1programmed cell death-1RECISTResponse Evaluation Criteria in Solid TumorsSAEssevere adverse eventsTMEtumor microenvironmentTregsregulatory T cells