antigen nucleotide and peptide sequence and MHC specificity (reactive T cell subtype [12])
| Antigen name | Reactive T cell subtype [12] | Nucleotide sequence in B16F1 cells | Peptide sequence |
|---|---|---|---|
| TRP2 | CD8 | AGCGTGTACGACTTCTTCGTGTGGCTG | SVYDFFVWL |
| Pbk | CD8 | GACAGCGGCAGCCCTTTTCCTGCTGCCGTGATCCTGAGAGATGCCCTGCACATGGCTAGAGGCCTGAAGTACCTGCACCAA | DSGSPFPAAVILRDALHMARGLKYLHQ |
| Kif18b | CD4 | CCTAGCAAGCCCAGCTTCCAAGAGTTCGTGGACTGGGAGAACGTGTCCCCTGAGCTGAACTCTACCGACCAGCCTTTCCTG | PSKPSFQEFVDWENVSPELNSTDQPFL |
| Cpsf3l | CD4 | GAGTTCAAGCACATCAAGGCCTTCGACAGAACCTTCGCTGACAACCCCGGACCTATGGTTCGACCTTGGCAGTCTGCTAGC | EFKHIKAFDRTFADNPGPMVRPWQSASSequence in B16F10 cells [12]: EFKHIKAFDRTFANNPGPMVVFATPGM |
| Gp100 | CD4 | TGGAACAGACAGCTGTACCCCGAGTGGACCGAGGCCCAGAGACTGGAT | WNRQLYPEWTEAQRL |
List of the antigens used in the study. Mutated amino acids of the peptide sequence compared to the wild type sequence are in bold and underlined in the table. Mutated amino acids of the gene Cpsf3l in the B16F10 cells compared to the wild type sequence are in bold and not underlined (the sequence in the table is the sequence found in B16F10 cells by Kreiter et al. [12])