Univariate analysis for overall survival

Univariate analysisMultivariate analysis
HR95% CIPHR95% CIP
Age (years)< 58 years10.913
≥58 years1.030.571.86
SexMale10.831
Female0.940.511.73
Disease presentationRecurrent10.327
Metastatic1.420.702.89
DifferentiationWD10.602
MD4.940.6139.76
PD4.410.5932.82
SRC3.410.4426.72
Others*3.640.3835.20
HER2Negative10.892
Positive0.810.341.93
Palliative chemotherapy historyNo10.00310.005
Yes4.191.6310.7810.782.0556.81
Molecular subtype of primary tumorMSI0.870.116.640.143
EBV19.111.90192.25
CIN-like1.010.492.07
GS-like1
Unknown1.330.632.84
VEGF-A (pg/mL)< 100.9 pg/mL10.127
≥100.9 pg/mL1.580.882.86
IL-10 (pg/mL)> 243.8 pg/mL10.16
≥243.8 pg/mL1.640.823.25
TGF-β1 (pg/mL)< 393.6 pg/mL10.197
≥393.6 pg/mL1.970.705.55
Cytokine-scoring model0–110.037
(number of high level of cytokines)2–31.911.043.49
CD4/CD3 (%)< 41.7%10.232
≥41.7%0.650.321.32
CD8/CD3 (%)< 33.3%10.272
≥33.3%1.580.703.57
CD4/CD8 ratio< 0.610.034
≥0.60.350.130.92
CD8CD45RO+/CD8 (%)< 67.2%10.02110.019
≥67.2%2.541.155.593.251.228.68
CD8CD25+/CD8 (%)< 1.6%10.081
≥1.6%1.920.923.99
CD8CD69+/CD8 (%)< 28.0%10.054
≥28.0%2.090.994.41
CD8HLA-DR+/CD8 (%)< 28.4%10.00810.012
≥28.4%3.771.439.965.361.4519.88
CD8 TIM3+/CD8 (%)< 0.2%10.082
≥0.2%0.500.231.09
CD8 LAG3+/CD8 (%)< 3.7%10.098
≥3.7%1.920.894.17
CD8 PD1+/CD8 (%)< 50.2%10.038
≥50.2%2.581.066.31
CD3 PD1+/CD3 (%)< 17.2%10.0011< 0.001
≥17.2%3.531.667.528.612.8126.37
CD4 PD1+/CD4 (%)< 32.1%10.052
≥32.1%2.110.994.50
FoxP3+ Tregs (No)< 38010.02910.001
≥3800.400.180.910.130.040.42
PD-L1 positive tumor cells (%)< 1%10.87
≥1%1.070.492.34

VEGF vascular endothelial growth factor, IL interleukin, TGF-β1 transforming growth factor- beta1, CD cluster of differentiation, WD well differentiated, MD moderate differentiated, PD poorly differentiated, SRC signet ring cell carcinoma, MSI microsatellite instability, EBV Epstein-Barr virus, CIN chromosomal instability, GS genomically stable, HR hazard ratio, CI confidence interval, P p-value, No number

Others*: mucinous adenocarcinoma, undifferentiated carcinoma