Table 1

Univariate and multivariate overall survival Cox proportional hazards regression including ICR cluster and stage, in all samples, ICR-enabled, ICR-disabled and ICR-neutral samples

Variables	Univariate		Multivariate		Stratified multivariate
Variables	HR (95% CI)	P value	HR (95% CI)	P value	HR (95% CI)	P value
ICR overall (n=4735)
ICR cluster (ICR low vs high)	1.203 (1.081 to 1.339)	0.00073***	1.232 (1.093 to 1.390)	0.00067***	1.217 (1.079 to 1.373)	0.00143**
Stage/grade	1.839 (1.731 to 1.954)	<2e-16***	1.838 (1.729 to 1.952)†	<2e-16***	Strata
Samples from ICR-enabled cancer types (n=1742)
ICR cluster (ICR low vs high)	1.631 (1.374 to 1.937)	2.26e-8***	1.488 (1.233 to 1.795)	3.35e-05***	1.494 (1.238 to 1.804)	2.94e-05***
Stage	1.817 (1.644 to 2.008)	<2e-16***	1.798 (1.628 to 1.987)†	<2e-16***	Strata
Samples from ICR-disabled cancer types (n=721)
ICR cluster (ICR low vs high)	0.6194 (0.480 to 0.799)	0.000229***	0.7702 (0.591 to 1.005)	0.0543	0.7253 (0.5527 to 0.9519)	0.0206*
Stage/grade	1.632 (1.418 to 1.879)	8.74e-12***	1.5703 (1.358 to 1.816)	1.12e-09***	Strata
Samples from ICR neutral cancer types (n=2272)
ICR cluster (ICR low vs high)	1.160 (0.983 to 1.369)	0.0789	1.247 (1.017 to 1.530)	0.034*	1.23 (1.001 to 1.51)	0.0485*
Stage/grade	1.944 (1.772 to 2.132)	<2e-16***	1.921 (1.751 to 2.108)†	<2e-16***	Strata

ICR cluster entered as categorical (factor) variable (factor levels: ‘ICR high’, ‘ICR low’). Stage is coded as stage I=1; stage II=2; stage III=3; stage IV=4. Histological grade was used for gliomas (LGG and GBM) instead of stage (LGG grade 2=2; LGG grade 3=3, grade 4 (GBM)=4). Only samples with all the variables available are included in the univariate and multivariate analyses.
*P<0.05, **P<0.01, ***P<0.001.
†Significant violation of proportional hazards assumption. HRs for death.
GBM, glioblastoma multiforme; ICR, immunological constant of rejection; LGG, lower grade glioma’s.