Affinity of mFcγRII and mFcRn for IgG subtypes
| FcγRII KD pH7.4 (M) | FcγRII KD pH6 (M) | Published FcRn KD pH6 (M) | KD ratio FcγRII/FcRn | |
| hIgG1 #1 | 3.02×10–6 | 4.92×10–8 | 7.2×10–8 | 0.68 |
| hIgG1 #2 | 6.61×10–6 | 7.90×10–8 | 7.2×10–8 | 1.10 |
| hIgG2 #1 | 7.65×10–6 | 2.10×10–7 | 6.3×10–8 | 3.33 |
| hIgG2 #2 | 1.45×10–5 | 2.18×10–7 | 6.3×10–8 | 3.46 |
| mIgG1 #1 | 2.74×10–7 | 2.77×10–9 | 1.57×10–6 | 0.0018 |
| mIgG1 #2 | 4.98×10–7 | 3.62×10–9 | 1.57×10–6 | 0.0023 |
| mIgG2a #1 | 1.18×10–6 | 2.36×10–8 | 4.9×10–7 | 0.054 |
| mIgG2a #2 | 1.10×10–6 | 2.41×10–8 | 4.9×10–7 | 0.049 |
Antibodies were immobilized on a Biacore CM5 chip before flowing mFcγRII or mFcRn over the chip. FcRn was used at pH6 with FcγRII at pH6 and pH7.4. KD was calculated using Biacore evaluation software.
FcγR, Fcγ receptors.