Table 1

Comparative immune T-cell reactivity against the 5T4 antigen as measured by functional assays in the blood and the prostate along with density of T-cell infiltration by IHC in 3 selected patients with ≥100% increase in serum PSA

Patient IDEx vivo ELISpot
magnitude of peak response (SFCs/106 PBMCs)
Flow cytometry on cultured PBMCs
peak response: fold increase over BL
Flow cytometry on expanded TILs
peak response: Δ% of IFN-γ+TNF-α+
IHC
immune infiltration compared with pre-vaccination tissue
PSA levels
% of maximum increase over BL
CD4+CD8+CD4+CD8+CD3+CD8+
VAN-0071025NDNDNDNDNY>100
VAN-02261 08.1NDNDNDND>100
VAN-02656 010.4 01.64YY>100
  • BL, baseline; IHC, immunohistochemistry; N, no; ND, not done; PBMC, peripheral blood mononuclear cell; PSA, prostate-specific antigen; SFC, spot-forming cell; TIL, tumor-infiltrating lymphocyte; Y, yes.