Table 2

Table of germ line and differentiation antigens from the Cancer Antigenic Peptide Database

Gene/protein	Tumor type	HLA restriction element	Peptide sequence	Reference	Total score mutant	MHC IC₅₀ (nM) mutant
Germ-line epitopes
BAGE-1	M	Cw16	AARAVFLAL	Boel, 1995	−0.53	419.6
CT37/FMR1NB	LC	A2	YLCSGSSYFV	Flores-Villanueva, 2018	0.3	8.5
Cyclin-A1	AML	A2	SLIAAAAFCLA	Ochsenreither, 2012	−2.08	894.4
Cyclin-A1	AML	A2	FLDRFLSCM	Ochsenreither, 2012	−0.46	29
GAGE-1,2,8	M	Cw6	YRPRPRRY	Van den Eynde, 1995	−0.26	1140.7
GAGE-3,4,5,6,7	M	A29	YYWPRPRRY	De Backer, 1999	1.65	13.1
GnTV	M	A2	VLPDVFIRC(V)	Guilloux, 1996	−1.41	438.5
HERV-E	RCC	A11	ATFLGSLTWK	Takahashi, 2008	0.21	7.8
HERV-K-MEL	M	A2	MLAVISCAV	Schiavetti, 2002	0.43	6.9
KK-LC-1	LC	B15	RQKRILVNL	Fukuyama, 2006	−0.6	517.9
KM-HN-1	EC	A24	NYNNFYRFL	Monji, 2004	−0.42	223.7
KM-HN-1	EC	A24	EYSKECLKEF	Monji, 2004	−0.35	774.8
KM-HN-1	EC	A24	EYLSLSDKI	Monji, 2004	−1.34	502.4
LAGE-1	M	A2	MLMAQEALAFL	Aarnoudse, 1999	1.01	11.7
LAGE-1	M	A2	SLLMWITQC	Rimoldi, 2000	−1.33	390.1
LAGE-1	Various	A31	LAAQERRVPR	Wang, 1998	−0.63	113.5
LAGE-1	M	B7	APRGVRMAV	Slager, 2004	0.61	3.3
LAGE-1	BC	A68	ELVRRILSR	Sun, 2006	−0.31	93
LAGE-1	M	B7	APRGVRMAV	Slager, 2004	0.61	3.3
LRPAP1	Various	A2	FLGPWAAS	Marijt, 2019	−3.82	5299.3
LY6K	Various	A24	RYCNLEGPPI	Suda, 2007	−1.37	519.5
MAGE-A1	M	A1	EADPTGHSY	Traversari, 1992	0.47	107.3
MAGE-A1	CC	A2	KVLEYVIKV	Pascolo, 2001	0.64	6.2
MAGE-A1	M	A3	SLFRAVITK	Chaux, 1999	0.14	16.2
MAGE-A1	BC	A2	KVLEYVIKV	Ottaviani, 2005	0.64	6.2
MAGE-A1	M	A68	EVYDGREHSA	Chaux, 1999	−2.92	7161.9
MAGE-A1	M	B7	RVRFFFPSL	Luiten, 2000	0.14	108.4
MAGE-A1	M	B35	EADPTGHSY	Luiten, 2000	1.24	16.1
MAGE-A1	M	B37	REPVTKAEML	Tanzarella, 1999	−1.49	3338.6
MAGE-A1	M	B44	KEADPTGHSY	Stroobant, 2012	0.59	144.9
MAGE-A1	M	B53	DPARYEFLW	Chaux, 1999	0.1	24.7
MAGE-A1	M	B57	ITKKVADLVGF	Corbière, 2004	−0.72	1775.4
MAGE-A1	M	Cw2	SAFPTTINF	Chaux, 1999	0.02	382.7
MAGE-A1	M	Cw3	SAYGEPRKL	Chaux, 1999	−0.43	219.7
MAGE-A1	MM	Cw7	RVRFFFPSL	Goodyear, 2011	−1.97	6638.8
MAGE-A1	M	Cw16	SAYGEPRKL	van der Bruggen, 1994	−0.75	525.8
MAGE-A10	M	A2	GLYDGMEHL	Huang, 1999	1.38	5.1
MAGE-A10	M	B53	DPARYEFLW	Chaux, 1999	0.1	24.7
MAGE-A12 m	M	A2	FLWGPRALV	van der Bruggen, 1994	0.14	11.1
MAGE-A12 m	BC	Cw7	VRIGHLYIL	Heidecker, 2000	−0.2	212.5
MAGE-A12 m	M	Cw7	VRIGHLYIL	Panelli, 2000	−0.2	212.5
MAGE-A12 m	M	Cw7	EGDCAPEEK	Breckpot, 2004	−3.8	43,575.9
MAGE-A2	M	A2	YLQLVFGIEV	Kawashima, 1998	−0.41	43.2
MAGE-A2	Various	A24	EYLQLVFGI	Tahara, 1999	−0.92	147.8
MAGE-A2	M	Cw7	EGDCAPEEK	Breckpot, 2004	−3.8	43,575.9
MAGE-A2	M	B37	REPVTKAEML	Tanzarella, 1999	−1.49	3338.6
MAGE-A3	M	A2	KVAELVHFL	Kawashima, 1998	0.98	11.2
MAGE-A3	M	A1	EVDPIGHLY	Gaugler, 1994	1.18	17.9
MAGE-A3	HNC	A24	TFPDLESEF	Oiso, 1999	−0.54	981.9
MAGE-A3	M	B18	MEVDPIGHLY	Bilsborough, 2002	0.94	46.1
MAGE-A3	M	B35	EVDPIGHLY	Schultz, 2001	0.51	74.6
MAGE-A3	M	B37	REPVTKAEML	Tanzarella, 1999	−1.49	3338.6
MAGE-A3	M	B44	MEVDPIGHLY	Herman, 1996	0.87	45.1
MAGE-A3	M	B40	AELVHFLLL	Schultz, 2002	0.6	19.9
MAGE-A3	M	B52	WQYFFPVIF	Russo, 2000	−0.52	1441.5
MAGE-A3	M	Cw7	EGDCAPEEK	Breckpot, 2004	−3.8	43,575.9
MAGE-A3	M	A2	FLWGPRALV	van der Bruggen, 1994	0.14	11.1
MAGE-A3	CRC	A24	VAELVHFLL	Miyagawa, 2006	−2.19	9428.9
MAGE-A4	Various	A2	GVYDGREHTV	Duffour, 1999	−1.47	971.6
MAGE-A4	M	A1	EVDPASNTY	Kobayashi, 2003	0.68	93.6
MAGE-A4	RCC	A24	NYKRCFPVI	Ottaviani, 2006	0	60.2
MAGE-A4	Various	A24	NYKRCFPVI	Miyahara, 2005	0	60.2
MAGE-A4	M	B37	SESLKMIF	Zhang, 2002	−1.68	11,466.1
MAGE-A6	M	B35	EVDPIGHVY	Benlalam, 2003	1.24	28.4
MAGE-A6	M	Cw7	EGDCAPEEK	Breckpot, 2004	−3.8	43,575.9
MAGE-A6	M	Cw16	ISGGPRISY	Vantomme, 2003	−0.11	628.4
#REF!	M	B37	REPVTKAEML	Tanzarella, 1999	−1.49	3338.6
MAGE-A9	RCC	A2	ALSVMGVYV	Oehlrich, 2005	−0.32	31.4
MAGE-C1	MM	A2	ILFGISLREV	Anderson, 2011	0.2	11.2
MAGE-C1	MM	A2	KVVEFLAML	Anderson, 2011	0.84	19.7
MAGE-C2	M	A2	ALKDVEERV	Ma, 2004	−1.26	342.2
MAGE-C2	M	B44	SESIKKKVL	Godelaine, 2007	−1.48	3608.4
MAGE-C2	M	A2	LLFGLALIEV	Ma, 2004	0.52	9.9
MAGE-C2	M	B57	ASSTLYLVF	Ma, 2011	0.31	226.5
NA88-A	M	B13	QGQHFLQKV	Moreau-Aubry, 2000	−3.07	16,389.9
NY-ESO-1/LAGE-2	M	A2	SLLMWITQC	Jäger, 1998	−1.33	390.1
NY-ESO-1/LAGE-2	M	A2	SLLMWITQC	Chen, 2000	−1.33	390.1
NY-ESO-1/LAGE-2	M	A2	SLLMWITQC	Valmori, 2000	−1.33	390.1
NY-ESO-1/LAGE-2	M	A2	MLMAQEALAFL	Aarnoudse, 1999	1.01	11.7
NY-ESO-1/LAGE-2	Various	A24	YLAMPFATPME	Eikawa, 2013	−4.36	32,521.3
NY-ESO-1/LAGE-2	Various	A31	ASGPGGGAPR	Wang, 1998	−1.4	498.5
NY-ESO-1/LAGE-2	Various	A31	LAAQERRVPR	Wang, 1998	−0.63	113.5
NY-ESO-1/LAGE-2	OC	A68	TVSGNILTIR	Matsuzaki, 2008	0.55	16.2
NY-ESO-1/LAGE-2	M	B7	APRGPHGGAASGL	Ebert, 2009	0.11	46.5
NY-ESO-1/LAGE-2	Various	B35	MPFATPMEAEL	Eikawa, 2013	−0.62	253.7
NY-ESO-1/LAGE-2	M	B49	KEFTVSGNILTI	Knights, 2009	−0.66	200.9
NY-ESO-1/LAGE-2	Various	B52	FATPMEAEL	Eikawa, 2013	−2.28	11,772.9
NY-ESO-1/LAGE-2	M	Cw3	LAMPFATPM	Gnjatic, 2000	0.48	2.6
NY-ESO-1/LAGE-2	M	Cw6	ARGPESRLL	Gnjatic, 2000	−1.38	2001
NY-ESO-1/LAGE-2	Various	C12	FATPMEAELAR	Eikawa, 2013	−2.63	16,931.9
NY-ESO-1/LAGE-2	M	B51	MPFATPMEA	Jäger, 2002	−2.09	704.3
SAGE	Various	A24	LYATVIHDI	Miyahara, 2005	−0.41	43.1
Sp17	MM	A1	ILDSSEEDK	Chiriva-Internati, 2003	−3.5	18,212.4
SSX-2	M	A2	KASEKIFYV	Ayyoub, 2002	0.01	16.6
TAG-1	M	A2	SLGWLFLLL	Adair, 2008	0.17	52
TAG-2	M	B8	LSRLSNRLL	Adair, 2008	−1.68	4019.9
TRP2-INT2	M	A68	EVISCKLIKR	Lupetti, 1998	0.52	19
XAGE-1b/GAGED2a	M	A2	RQKKIRIQL	Ohue, 2012	−2.19	19,497
Differentiation epitopes
CEA	GIC	A2	YLSGANLNL	Tsang, 1995	0.71	18.8
CEA	GIC	A24	TYACFVSNL	Nukaya, 1999	−0.05	60.1
CEA	GIC	A2	GVLVGVALI	Alves, 2007	−1.64	637.3
CEA	GIC	A3	HLFGYSWYK	Kawashima, 1999	0.17	7.9
CEA	GIC	A24	QYSWFVNGTF	Nukaya, 1999	1.26	17
CEA	GIC	A2	IMIGVLVGV	Kawashima, 1998	0.78	4.6
gp100/Pmel17	M	A2	KTWGQYWQV	Bakker, 1995	0.46	11.8
gp100/Pmel17	M	A2	MLGTHTMEV	Tsai, 1997	0.33	7.5
gp100/Pmel17	M	A2	KTWGQYWQV	Kawakami, 1995	0.46	11.8
gp100/Pmel17	M	A2	ITDQVPFSV	Kawakami, 1995	−1.2	211.5
gp100/Pmel17	M	A2	YLEPGPVTA	Cox, 1994	−1.09	172.7
gp100/Pmel17	M	A2	VLYRYGSFSV	Kawakami, 1995	0.12	12.3
gp100/Pmel17	M	A2	LLDGTATLRL	Kawakami, 1994	−0.51	347.7
gp100/Pmel17	M	A2	RLMKQDFSV	Kawakami, 1998	0.66	4.9
gp100/Pmel17	M	A2	SLADTNSLAV	Tsai, 1997	−0.32	47.7
gp100/Pmel17	M	A3	LIYRRRLMK	Kawakami, 1998	0.53	6.1
gp100/Pmel17	M	A2	RLPRIFCSC	Kawakami, 1998	−2.06	1491.6
gp100/Pmel17	M	A3	IALNFPGSQK	Kawashima, 1998	−0.19	31
gp100/Pmel17	M	A3	ALLAVGATK	Skipper, 1996	−0.35	45.2
gp100/Pmel17	M	A3	RSYVPLAHR	Michaux, 2014	0.3	68
gp100/Pmel17	M	A3	ALNFPGSQK	Kawashima, 1998	−0.03	19
gp100/Pmel17	M	A24	VYFFLPDHL	Robbins, 1997	−0.05	77.6
gp100/Pmel17	M	A11	ALNFPGSQK	Kawashima, 1998	−0.67	46.7
gp100/Pmel17	M	B7	SSPGCQPPA	Lennerz, 2005	−3.78	23,794.9
gp100/Pmel17	M	A68	HTMEVTVYHR	Sensi, 2002	1.34	2.6
gp100/Pmel17	M	A32	RTKQLYPEW	Vigneron, 2004	−0.02	69
gp100/Pmel17	M	B35	VPLDCVLYRY	Benlalam, 2003	0.23	154.7
gp100/Pmel17	M	B35	LPHSSSHWL	Vigneron, 2005	−0.73	240.8
gp100/Pmel17	M	Cw8	SNDGPTLI	Castelli, 1999	−3.07	31,048.6
mammaglobin-A	BC	A3	PLLENVISK	Jaramillo, 2002	−2.42	2471.7
Melan-A/MART-1	M	A2	ILTVILGVL	Castelli, 1995	−0.6	529.6
Melan-A/MART-1	M	A2	AAGIGILTV	Kawakami, 1994	−2.13	3674
Melan-A/MART-1	M	Cw7	RNGYRALMDKS	Larrieu, 2008	−4.73	42,544.4
Melan-A/MART-1	M	B35	EAAGIGILTV	Benlalam, 2003	−3.08	15,289.2
Melan-A/MART-1	M	A2	SLSKILDTV	Wang, 2006	0.04	15.1
NY-BR-1	BC	A24	LYSACFWWL	Touloukian, 2003	0.04	91.7
OA1	M	A2	TLMSAMTNL	Olson, 2010	0.89	15.3
PAP	PC	A2	ALDVYNGLL	Olson, 2010	−0.66	240.5
PAP	PC	A2	FLFLLFFWL	Olson, 2010	0.22	42.5
PAP	PC	A2	FLTPKKLQCV	Correale, 1997	−0.84	126.4
PSA	PC	A2	VISNDVCAQV	Correale, 1997	−1.21	359
PSA	PC	A2	VLHWDPETV	Walton, 2006	−0.58	116.7
RAB38/NY-MEL-1	M	A31	MSLQRQFLR	Wang, 1996	0.77	5.4
TRP-1/gp75	M	A2	SVYDFFVWL	Parkhurst, 1998	0.87	21.6
TRP-2	M	A2	TLDSQVMSL	Noppen, 2000	0.26	52.5
TRP-2	M	A31	LLGPGRPYR	Wang, 1996	0.27	24.1
TRP-2	M	Cw8	ANDPIFVVL	Castelli, 1999	−1.2	2060.1
TRP-2	M	A33	LLGPGRPYR	Wang, 1998	−0.47	115.1
TRP-2	M	A1	KCDICTDEY	Kittlesen, 1998	−0.62	1397.5
tyrosinase	M	A1	SSDYVIPIGTY	Kawakami, 1998	0.43	147.3
tyrosinase	M	A2	MLLAVLYCL	Wölfel, 1994	1.21	8.3
tyrosinase	M	A2	CLLWSFQTSA	Riley, 2001	−1.08	99.6
tyrosinase	M	A2	YMDGTMSQV	Wölfel, 1994	0.47	5.7
tyrosinase	M	A2	YMDGTMSQV	Skipper, 1996	0.47	5.7
tyrosinase	M	A24	IYMDGTADFSF	Dalet, 2011	1.09	23.4
tyrosinase	M	A24	AFLPWHRLF	Kang, 1995	0.85	44.6
tyrosinase	M	A26	QCSGNFMGF	Lennerz, 2005	−1.83	14,811.2
tyrosinase	M	B35	LPSSADVEF	Morel, 1999	1.82	4.1
tyrosinase	M	B35	TPRLPSSADVEF	Benlalam, 2003	−1.07	2534.5
tyrosinase	M	B38	LHHAFVDSIF	Lennerz, 2005	−0.48	620.8
tyrosinase	M	B44	SEIWRDIDF	Brichard, 1996	−0.16	250.7

Germ-line antigens are expressed in many tumors but not in normal tissues. Differentiation antigens are also expressed in the normal tissue of origin of the malignancy. Total score and MHC IC₅₀ were predicted using the IEDB proteasomal cleavage/TAP transport/MHC class I combined predictor tool for mutant and wild-type peptides. Each line corresponds to a peptide that is considered to be a tumor antigen that is recognized by T cells. For each antigenic peptide, evidence of natural processing and presentation and isolation of stable human T cell clones that recognize the peptide were required for inclusion in the table. The MHC I binding predictions were made on March 27, 2020 using the IEDB Analysis Resource Consensus Tool94 that combines predictions from ANN a.k.a. NetMHC,95–97 SMM,98 and Comblib.99
HLA, human leucocyte antigen; IEDB, immune epitope database; MHC, major histocompatibility complex; TAP, transporter associated with antigen processing.