Table 4

Proposed allocation of bone tissues for various analyses

Portion of 1 cm3 sample (%)CoresPreservation conditionPotential uses
201–2 vs touch prepFrozen sectionConfirm viable tissue
202FFPEHistology, IHC, FISH
101Flash frozenRT-PCR
201–2RPMICytogenetics, FISH, flow cytometry
30 (when possible)2–3 when possibleFrozenBanking
  • FFPE, formalin-fixed paraffin-embedded; FISH, Fluorescence in situ hybridization; RPMI, Roswell Park Memorial Institute; RT-PCR, reverse transcription PCR.