Portion of 1 cm3 sample (%) | Cores | Preservation condition | Potential uses |
20 | 1–2 vs touch prep | Frozen section | Confirm viable tissue |
20 | 2 | FFPE | Histology, IHC, FISH |
10 | 1 | Flash frozen | RT-PCR |
20 | 1–2 | RPMI | Cytogenetics, FISH, flow cytometry |
30 (when possible) | 2–3 when possible | Frozen | Banking |
FFPE, formalin-fixed paraffin-embedded; FISH, Fluorescence in situ hybridization; RPMI, Roswell Park Memorial Institute; RT-PCR, reverse transcription PCR.