Table 1

List of dual regulators (left) and cytotoxicity regulators (right) identified by the screens under two different types of selection pressure

Dual immune resistance regulators			Cytotoxicity resistance regulators
Gene	log₂ FC	Adjusted p value	Gene	log₂ FC	Adjusted p value
ALDOA	−0.23850373	0.007872359	BIRC5	0.001616317	0.445455851
ATG14	−0.16059775	0.024714023	CENPM	−0.02500863	0.490792818
BCS1L	−0.2221878	0.000232251	CHMP3	−0.04904437	0.288401697
CDC37	−0.13924483	0.013138636	CUL1	0.043406838	0.357618622
CINP	−0.27976923	0.00013675	GPN2	−0.02216915	0.436860262
EMC6	−0.17631624	0.003022816	GPN3	0.054197902	0.490792818
IARS2	−0.19416706	0.024714023	GTF2A2	−0.1117453	0.129400101
INTS1	−0.37983256	0.000232251	IKBKB	0.023644056	0.468032515
LAGE3	−0.23234266	0.003485045	IKBKG	−0.01643131	0.497356193
MRPL24	−0.40095843	5.04E-07	MAP3K7	0.103247863	0.161295174
MRPL34	−0.16222743	0.032171236	MED14	0.04430575	0.405754176
MRPS18A	−0.1803627	0.003485045	MED20	−0.16001865	0.051695527
MRPS25	−0.27447257	0.001960487	MED28	0.014100544	0.479837582
NDUFA13	−0.24127607	0.00077217	MED31	−0.00650653	0.431123004
NDUFA8	−0.14254375	0.036841903	MED4	0.029429293	0.490792818
NDUFC2	−0.22780474	0.009719995	MED8	0.025281119	0.469465067
NDUFS2	−0.27413473	0.000150695	NEDD8	−0.10738096	0.057838148
NDUFS8	−0.21857521	0.010858763	OSGEP	−0.09611608	0.210488175
NELFCD	−0.13824149	0.021162677	PABPN1	−0.04169503	0.369604337
NFRKB	−0.14577584	0.048562248	PISD	−0.00640109	0.445455851
OXA1L	−0.24881438	0.000150695	POLR2C	−0.04248415	0.389528608
PFDN2	−0.20551461	0.010483911	POLR3B	−0.09054623	0.143058435
PHB	−0.17979782	0.017985676	PRC1	−0.03823675	0.469573522
POLR2A	−0.16735144	0.036239233	RBCK1	0.002557887	0.478498029
POLR2F	−0.18849316	0.017322046	RELA	−0.12211608	0.057838148
POLR2J	−0.18380023	0.005798006	RIPK1	−0.0370495	0.368548703
POLR2L	−0.21763683	0.01732348	RPP14	−0.02148104	0.226554319
PPP2R1A	−0.19027009	0.003022816	SIL1	−0.09891158	0.161295174
PRMT1	−0.20861375	0.003149464	STRAP	−0.11208718	0.105544333
QARS	−0.19534141	0.000879503	SUPT16H	−0.1103425	0.1585083
RNF31	−0.12674219	0.048562248	TAB1	−0.10182075	0.077284946
RPL8	−0.21557521	0.020928307	UBA3	0.125112432	0.1585083
RPS2	−0.22193232	0.009801511	VMP1	0.079953225	0.304995581
SF3A2	−0.27189946	0.001678396	WDR1	−0.02369511	0.392659421
SHARPIN	−0.21693714	0.003149464	CAPZB	0.170981197	0.005016426
SMARCA4	−0.24420365	0.000504813	CFLAR	0.49050373	2.26E-07
SUPT5H	−0.11043007	0.040195832
TAF6	−0.18760288	0.00306836
TFPT	−0.23112836	0.006997517
THRA	−0.31436908	0.000747174
TIMM22	−0.18787382	0.000747174
TMEM161	−0.31722681	1.74E-05
TONSL	−0.2265871	0.030279691
TOP3A	−0.14123831	0.029872015
TUFM	−0.14949456	0.007083264
USP5	−0.22581678	0.000303673
VPS25	−0.16491795	0.007165671
VPS28	−0.20945587	0.002049957

Genes whose gRNAs were significantly depleted in the T cell-treated group were aligned with their related human homologs. Based on the cut-off of lymphocyte infiltration scores (L scores), patients with melanoma in the TCGA dataset into high L score (L score >3) and low L score (L score ≤3) groups. The expression levels of selected genes between the high L score group versus the low L score group were compared. The Log₂FC (high vs low) and adjusted p value for each candidate were calculated. Dual regulators are defined as genes with adjusted p<0.05 and Log₂FC<0. Cytotoxicity regulators are defined as genes with either adjusted p≥0.05 or Log₂FC≥0. The regulators identified by both selection conditions were highlighted in yellow. The cytotoxicity regulators identified under single selection condition were not shown.