Receptor affinity of rhIL-15, NKTR-255 and rhIL-15/IL-15Rα
| IL-15Rα | IL-2Rβ | |||||
| kon (M-1sec-1) | koff (sec-1) | KD (pM) | kon (M-1sec-1) | koff (sec-1) | KD (nM) | |
| rhIL-15 | 5.1±1.4×106 | 9.3±0.97×10-5 | 19±3.4 | 6.6±0.54×105 | 0.036±0.0077 | 59±16 |
| NKTR-255 | 7.8±0.74×105 | 6.7±1.7×10-5 | 85±14 | 1.3±0.18×105 | 0.034±0.0023 | 260±52 |
| rhIL-15/IL-15Rα | ND | ND | ND | 4.0±1.9×106 | 0.025±0.00099 | 6.6±0.86 |
Human IL-15Rα or IL-2Rβ were captured on a Biacore surface plasmon resonance sensor chip and relative affinities were calculated from kinetic measurements. Each assay was performed in duplicate. KD, dissociation constant (affinity); koff, mean dissociation rate constant; kon, mean association rate; ND, not determined.
IL, interleukin; rhIL-15, recombinant human IL-15.