Table 2

PD-L1 molecule numbers on SKOV3 or OVCAR3 cells 24 hours after exposure to primary T cells (10:1 E:T) and various IgGs and IgG BsAbs (0.1 nM). Columns with ‘fresh’ tumor cells were incubated for 24 hours with supernatants from the original redirected lysis assays cultures (T cells and original tumor cells removed by centrifugation), thus evaluating whether factors released into the supernatants of an active redirected lysis assay could induce PD-L1 upregulation on untreated tumor cells

Test articlePD-L1 molecules per cell surface (SKOV3 cells+T cells)PD-L1 molecules per cell surface (fresh SKOV3 cells+24-hour supernatant)PD-L1 molecules per cell surface (OVCAR3 cells+T cells)PD-L1 molecules per cell surface (fresh OVCAR3 cells+24-hour supernatant)
IgG1 Cntrl3500710045001600
SP34 IgG1_N297Q7700570020001800
Pertuzumab IgG13900300020001600
SP34+Pertuz IgGs8300620022001800
SP34/Pertuz IgG BsAb29 30029 40059003000
SP34_F100fH/Pertuz BsAb24 30027 30050002900
  • BsAb, bispecific antibody; PD-L1, Programmed cell death-ligand 1; scFV, Single chain variable fragment.