Table 2

DBY wildtype and mutated variant peptides

Peptide	Sequence	NetMHCII 2.2. prediction
Peptide	Sequence	Binding	Affinity (nM)
WT	YRQSSGSANA^-2GF¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	Weak	158
A10F/N15A	YRQSSGSANF^-2GF¹NSA⁴RA⁶NSS⁹RSSGSSHNRG	Strong	17
F12W	YRQSSGSANA^-2GW¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	569
A10F/F12A	YRQSSGSANF^-2GA¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	1030
F12I	YRQSSGSANA^-2GI¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	1361
F12M	YRQSSGSANA^-2GM¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	2182
F12A	YRQSSGSANA^-2GA¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	2321
F12A/A17P	YRQSSGSANA^-2GA¹NSN⁴RP⁶NSS⁹RSSGSSHNRG	–	2460
F12A/R20M	YRQSSGSANA^-2GA¹NSN⁴RA⁶NSM⁹RSSGSSHNRG	–	2608
F12A/R20T	YRQSSGSANA^-2GA¹NSN⁴RA⁶NST⁹RSSGSSHNRG	–	2852
A10F/F12A/N18L	YRQSSGSANF^-2GA¹NSN⁴RA⁶LSS⁹RSSGSSHNRG	–	3301
F12G	YRQSSGSANA^-2GG¹NSN⁴RA⁶NSS⁹RSSGSSHNRG	–	5440
F12A/A17I	YRQSSGSANA^-2GA¹NSN⁴RI⁶NSS⁹RSSGSSHNRG	–	5862

Anchor amino acids of the core peptide region which possibly interact with the I-A^b restriction molecule are indicated by numbers. Mutated amino acids are highlighted. Binding strength is characterized by the binding affinity as calculated by the NetMHCII 2.2 software (strong <50 nM; weak <500 nM). Peptide variants that did not induce any T-cell recognition are shaded in gray.