Table 1

Ovalbumin wildtype and mutated variant peptides

Peptide	Sequence	NetMHCII 2.2 prediction
Peptide	Sequence	Binding	Affinity (nM)
WT	ISQAV^-2HA¹AHAEI⁶NEAGREVVGSAEAGVDAA	weak	339
I334A	ISQAV^-2HA¹AHAEA⁶NEAGREVVGSAEAGVDAA	weak	89
A329Y/I334A	ISQAV^-2HY¹AHAEA⁶NEAGREVVGSAEAGVDAA	strong	37
V327F/A329Y/I334A	ISQAF^-2HY¹AHAEA⁶NEAGREVVGSAEAGVDAA	strong	14

Anchor amino acids of the core peptide region which possibly interact with the I-A^b restriction molecule are indicated by numbers. Mutated amino acids are highlighted. Binding strength is characterized by the binding affinity as calculated by the NetMHCII 2.2 software (strong <50 nM; weak <500 nM).