Table 1

Characteristics of neoantigens targeted by identified TCRs

Peptide nameGene name Ensembl transcript IDPeptide sequenceHLA restrictionPredicted affinity (nM), rank (%)Experimental IC50 (nM)MS FDR (%)Reads exome PBMCs (Ref:Alt)Reads exome MInt (Ref:Alt)Reads exome MLung (Ref:Alt)
SYTL4S363FSYTL4; ENST00000263033GRIAFFLKYB27:0518.43; 0.6214.7±1.8154:032:1071:20
SYTL4WTGRIAFSLKY30.62; 0.1297.32±2.1
KIF2CP13LKIF2C; ENST00000372224RLFLGLAIKA03:0121.6; 0.09237.4±2.075:075:18177:21
KIF2CWTRLFPGLAIK12.10; 0.03146.2±1.1
NCAPG2P333LNCAPG2; ENST00000409339KLILWRGLKA03:0132.6; 0.15202.9±1.031126:0146:2754:0
NCAPG2WTKPILWRGLK6946.48; 6.504,488.7±10.4
  • Alt, alternative read; FDR, false discovery rate; HLA, human leukocyte antigen; IC50, half maximal inhibitory concentration; MInt, intestinal metastasis; MLung, lung metastasis; PBMC, peripheral blood mononuclear cell; Ref, reference read; TCR, T-cell receptor.