Table 1

Antibodies used in this study

Antibody	Clone	Fluorophore	Source	Dilution
Anti-mCD28	37.51	Unconjugated	BioLegend	–
Anti-mCD3ε	145–2 C11	Unconjugated	BioLegend	–
Anti-mCD3ε	145–2 C11	PerCP-Cy5.5	BioLegend	1:200
Anti-mCD45	30-F11	BV570	BioLegend	1:200
Anti-mNK1.1	PK136	PE	BioLegend	1:100
Anti-mCD8	53–6.7	Alexa Fluor 700	BioLegend	1:200
Anti-mCD8	53–6.7	Alexa Fluor 647	BioLegend	1:200
Anti-mCD4	GK1.5	APC-Cy7	BioLegend	1:200
Anti-m/hGzmB	QA16A02	PE	BioLegend	1:100
Anti-mIFN-γ	XMG1.2	PE-Cy7	BioLegend	1:100
Anti-mTNF-α	MP6-XT22	PE-Dazzle	BioLegend	1:100
Anti-mCD69	H1.2F3	PE-Cy7	BioLegend	1:200
Anti-m/hCD44	IM7	BV711	BioLegend	1:200
Anti-mCD19	6D5	BV785	BioLegend	1:100
Anti-mGr1	RB6-8C5	APC/Cy7	BioLegend	1:400
Anti-mCD11b	M1/70	Pacific blue	BioLegend	1:100
Anti-mF4/80	BM8	PECy7	BioLegend	1:100
Anti-mCD11c	N418	PerCP Cy5.5	BioLegend	1:100
Anti-mMHCII	M5/114.15.2	AF700	BioLegend	1:400
Anti-mCD103	2E7	APC	BioLegend	1:200
Anti-mCD86	GL-1	PE	BioLegend	1:200
Anti-mCD80	16-10A1	BV650	BioLegend	1:100
Anti-mCD45.1	A20	PerCPCy5.5	BioLegend	1:200
Anti-mIDO1	2E2/IDO1	AF647	BioLegend	1:200
Anti-mPD-L1	10F.9G2	BV650	BioLegend	1:100
Anti-mPD-L2	Ty25	PE-Dazzle	BioLegend	1:200
Anti-mCD70	FR70	APC	BioLegend	1:200
Anti-mCD137L	TKS-1	PE	BioLegend	1:200
Anti-mCD40	3/23	PE-Dazzle	BioLegend	1:200
Anti-mOX40L	RM134L	APC	BioLegend	1:200
Anti-mIL-12	C17.8	PE	BioLegend	1:200
Anti-mCD25	PC61	BV650	BioLegend	1:200
Anti-mCTLA4	UC10-4B9	PE-Dazzle	BioLegend	1:100
Anti-mGITR	YGITR 765	eFluor450	BioLegend	1:100
Anti-mFoxp3	FJK-16s	FITC	Thermo Fisher Scientific	1:100
Anti-rabbit IgG		Alexa Fluor 647	Thermo Fisher Scientific	1:500
Anti-rat IgG		Alexa Fluor 555	Thermo Fisher Scientific	1:500
Anti-mCD4	GK1.5		Thermo Fisher Scientific (MA1-146)	1:100
Anti-mCD8	4SM15		Thermo Fisher Scientific (14–0808)	1:100
Anti-mFoxp3			Abcam (ab54501)	1:100
Anti-mPKCη	RabMab EPR18513		Abcam (ab179524)	1:500

h, human; m, mouse.