Table 1

Prognostic relevance of T lymphocytes, dendritic cells (DCs), tertiary lymphoid structures (TLSs) and B cells in primary and metastatic ovarian carcinoma

	Histology	Stage	No of patients	Method	Impact	Note	Ref.
T lymphocytes
Primary lesion
CD3⁺ T cell	EOC*	III, IV	174	IHC, IF	Beneficial	High density of intratumoral TILs correlated with improved survival	40
CD3⁺ T cell	HGSOC	All	199	IHC	Beneficial	High density of CD3⁺ T cells correlated with improved disease outcome	3
CD8⁺ T cell	EOC	All	270	IHC	Beneficial	High density of CD8⁺ T cells correlated with favorable disease outcome	29
	EOC	All	117	IHC	Beneficial	High density of CD8⁺ T cells correlated with favorable prognosis	30
	EOC	All	500	IHC	Beneficial	High density of CD8⁺ T cells associated with low BRCA1 expression	41
	EOC	All	1815	Meta-analyses	Beneficial	High density of CD3⁺ and CD8⁺ T cells correlated with improved disease outcome	28
	EOC	All	497	IHC, IF	Beneficial	High density of CD103⁺CD8⁺ T cells correlated with improved survival	33
	EOC	II, III, IV	203	IHC, RT-PCR	Beneficial	High density of CD8⁺ T cells correlated with favorable disease outcome	34
	EOC	All	70	IHC	Beneficial	CD8⁺ T cells negatively correlated with PD-L1 expression in tumor	35
	EOC	All	199	IHC	Beneficial	High density of CD8⁺ T cells correlated with improved disease outcome	3
	HGSOC, EnOC, OCCC	All	135	IHC	Beneficial	High density of CD103⁺CD8⁺ T cells correlated with improved disease outcome	22
	EOC	All	7377	IHC	Beneficial	High density of CD8⁺ T cells correlated with prolonged OS	44
	EOC	All	210	IHC	Beneficial	High density of intratumoral CD8⁺ CTLs correlated with favorable disease outcome	37
	HGSOC	III	100	IHC	Beneficial	High density and clonal selection of TILs correlated with improved disease survival	31
	HGSOC	All	178	IHC	Beneficial	CD8⁺ T cells were shown to abolish clinically relevant chemoresistance by altering glutathione and cystine metabolism in malignant cells	32
	HGSOC	All	147	IHC	Beneficial	High density of CD8⁺ T cells correlated with improved disease outcome	21
	HGSOC	All	232	IHC	Beneficial	High density of CD8⁺ T cells correlated with improved disease outcome	38
	HGSOC	All	283	IHC	Beneficial	High density of CD8⁺ T cells correlated with favorable prognosis	39
	HGSOC	All	80	IF	Detrimental	High density of PD-1⁺TIM-3⁺CD8⁺ T cells correlated with poor disease outcome	45
CD45RO⁺ T cell	EOC	All	270	IHC	Beneficial	High density of CD45RO⁺ memory T cells correlated with increased disease specific survival	29
CD45RO⁺ T cell	EOC	All	33	IHC	Beneficial	High density of CD45RO⁺ T cells correlated with higher survival rate	42
T_REG cell	EOC	All	270	IHC	Beneficial	High density of T_REG cells correlated with favorable disease outcome	29
	EOC	All	92	IHC, FC	Detrimental	High density of CD8⁺ T_REG cells, induced by TGFB1, correlated with poor disease outcome	66
	EOC	All	232	IHC	Detrimental	High density of T_REG cells associated with advanced stage of disease and suboptimal debulking	38
	EOC	III,IV	26	IHC,FC	Detrimental	High density of ICOS⁺ T_REG cells, dependent on ICOS-L stimulation by pDC, correlated with poor disease outcome	17
	EOC	All	103	IF	Detrimental	High density of T_REG cells, inducing the expression of B7-H4 on TAMs, correlated with poor disease outcome	56
	EOC	All	70	IF	Detrimental	High density of T_REG cells in tumor and malignant ascites was associated with increased production of CCL22 by cancer cells and TAMs	54
	EOC	All	869	Meta-analyses	Detrimental	High density of T_REG cells correlated with poor disease outcome	55
	EOC	All	210	IHC	Detrimental	High density of T_REG cells in lymphoid aggregates correlated with reduced survival time	37
	HGSOC	All	199	IHC	Beneficial	High density of T_REG cells correlated with improved disease outcome	3
	HGSOC, MOC, Other	All	99	IHC, RT-PCR	Detrimental	High density of T_REG cells correlated with poor OS and PFS	53
	HGSOC, MOC, OCCC	All	25	FC,RNAseq, Cytof	Detrimental	High density of T_REG cells, suppressing CD8⁺ T cells proliferation, correlated with poor disease outcome	62
CD8/T_REG	EOC	All	270	IHC	Beneficial	High ratio of CD8⁺ T cells^/T_REG cells correlated with improved disease outcome	29
	EOC	All	117	IHC	Beneficial	High CD8⁺ T cells/T_REG cells ratio correlated with favorable prognosis	30
	EOC	All	400	IHC,IF	Beneficial	High CD8⁺ T cells/T_REG cells ratio correlated with favorable prognosis	57
T_REG/T_H17	EOC	III, IV	124	IF	Detrimental	High T_REG cells/T_H17⁺ cells ratio, derived by TAMs, associated with disease progression and metastasis	60
Metastatic lesion
CD8⁺ T cell	EOC	III, IV	147	IHC	Beneficial	High density of CD8⁺ CTLs in omental metastasis correlated with improved disease specific survival	29
CD8⁺ T cell	HGSOC	III, IV	80	IHC	None	High density of CD8⁺ T cells in peritoneal metastasis were not shown to associate with disease outcome	20
CD45RO⁺ cell	EOC	III, IV	147	IHC	Beneficial	High density of CD45RO⁺T cells in omental metastasis correlated with improved disease specific survival	29
CD45RO⁺ cell	HGSOC	III, IV	77	FC	Beneficial	High density of CD45RA^-CCR7^-CD8⁺ T cells in peritoneal ascites correlated with improved RFS	43
T_REG cell	EOC	III, IV	147	IHC	Detrimental	High density of T_REG cells in omental ascites, mediated by CCL22 produced by cancer cells and TAMs, correlated with poor disease outcome	29
CD8/T_REG	EOC	III, IV	147	IHC	Detrimental	High CD8⁺ T cells/T_REG cells ratio in omental metastasis correlated with poor disease specific survival	29
DCs
Primary lesion
mDC	*EOC	All	33	IHC	Beneficial	High density of CD1a⁺ DCs correlated with improved OS	42
	n.a.	n.a.	n.a.	FC, IHC	Benefical	High level of CXCL17 correlated with increased density of B7-H4⁺ DCs and favorable disease outcome	74
	n.a.	n.a.	n.a.	FC, IHC	Beneficial	High density of CD103⁺ DCs, as potent stimulators of CTLs, correlated with favorable disease outcome	72
	HGSOC	All	147	IHC	Benefical	High density of mature DC-LAMP⁺ DCs correlated with increased frequency of CTLs and improved disease outcome	21
pDC	n.a.	All	44	FC	Detrimental	High density of CD4⁺CD123⁺BDCA2⁺ pDC correlated with poor disease outcome	77
	EOC	All	60	IHC, FC	Detrimental	High expression of IDO correlated with reduced density of CD8⁺ T cells, tumor progression and poor disease outcome	78
	n.a.	n.a.	n.a.	IHC, FC	Detrimental	SDF-1 was shown to induce chemotaxis and protection of pDCs from TAM-mediated apoptosis	79
	EOC	III, IV	26	IHC, FC	Detrimental	High density of HLA-DR⁺CD123⁺ pDC mediated stimulation of ICOS⁺ T_REG cells associated with poor disease outcome	17
Metastatic lesion
mDC	HGSOC	III, IV	80	IHC	Beneficial	High density of mature DC-LAMP⁺ DCs in peritoneal metastasis correlated with improved disease outcome	20
pDC	n.a.	All	44	FC	Detrimental	High density of CD4⁺CD123⁺BDCA2⁺ pDCs in malignant ascites correlated with poor disease outcome	77
TLSs and B cells
Primary lesion
B cell	*EOC	All	135	IHC	Beneficial	High density of CD20⁺ B cells correlated with improved disease outcome	36
	EOC	All	266	RNAseq	Beneficial	High BCR segments correlated with improved prognosis	82
	EOC	All	154	IHC	Detrimental	High density of CD138⁺ plasma cells correlated with poor disease outcome	85
	HGSOC, EnOC	All	224	RNAseq	Beneficial	High expression of CD38 correlated with favorable prognosis	84
	HGSOC	All	194	IHC	Beneficial	High density of CD27^-CD20⁺ memory B cells correlated with cytolytic immune response and favorable prognosis	81
	HGSOC	All	147	IHC	Beneficial	High density of CD20⁺ B cells correlated with CTLs response and improved RFS and OS	21
	HGSOC	All	199	IHC	Beneficial	High density of CD20⁺ B cells correlated with favorable disease outcome	3
	HGSOC	All	155	IHC	Beneficial	High density of CD20^-CD38⁺CD138⁺CD79a⁺ plasma cells correlated with CTLs response and improved disease outcome	83
TLS	HGSOC	All	147	IHC	None	The presence of TLSs was not associated with disease outcome	21
	HGSOC	All	155	IHC	Beneficial	TLSs were shown to facilitate the development of antitumor immunity associated with favorable disease outcome	83
Metastatic lesion
B cell	HGSOC	III, IV	80	IHC	None	High density of CD20⁺ B cells in peritoneal metastasis was not associated with disease outcome	20
	HGSOC	III, IV	41	IHC	Beneficial	High density of memory 20⁺ B cells in omental metastasis correlated with cytolytic immune response and favorable disease outcome	89

*Encompassing all EOC histological subtypes.
CTL, cytotoxic T lymphocyte; CXCL9, C-X-C motif chemokine ligand 9; EnOC, endometroid ovarian cancer; EOC, epithelial ovarian carcinoma; FC, flow cytometry; HGSOC, high-grade serous ovarian carcinoma; ICOS, inducible T cell costimulator; IDO, indoleamine 2,3-dioxygenase; IF, immunofluorescence; IHC, immunohistochemistry; LAMP, lysosomal-associated membrane protein; mDC, myeloid DC; MOC, mucinous ovarian cancer; n.a., not available; OCCC, clear cell ovarian cancer; OS, overall survival; PD-1, programmed cell death 1; pDC, plasmacytoid DC; PFS, progression-free survival; RFS, relapse-free survival; RNAseq, RNA-sequencing; TAM, tumor-associated macrophage; TGFB1, transforming growth factor beta 1; TIL, tumor-infiltrating lymphocyte; T_REG, regulatory T cell.