Table 1

Baseline clinical characteristics and immunologic findings of the pre- and post-CAR-T-cell therapy cohorts

Demographics			Diagnosis and treatments prior to CAR-T-cell therapy			CAR-T-cell therapy and vaccine				Baseline immunologic findings*
Study ID	Age group, years	Sex	Underlying diagnosis†	Prior HCT, months to vaccine	mAb within prior 6 months before vaccine‡	CAR-Tx target§	Months, CAR-Tx to vaccine	Vaccine type¶	IgG, mg/dL	CD19+ B cells /µL	CD4+ T cells /µL
Pre-CAR-T cohort
Individuals with an antibody response to ≥1 vaccine strain
Pre-4	18–60	F	MM		yes	BCMA		IIV4	21	21.0	470
Pre-5	61–75	M	MM	Auto, 43	yes	BCMA		IIV4	591	87.2	436
Individuals without antibody responses
Pre-1	18–60	M	ALL		yes	CD19		IIV4	376	4.7	236
Pre-2	18–60	M	NHL	Auto, 19		CD20		IIV4	552	<0.1	103
Pre-3	18–60	M	MM	Auto, 32		BCMA		IIV4	46	47.2	433
Post-CAR-T cohort
Individuals with an antibody response to ≥1 vaccine strain
Post-1	18–60	M	ALL	Allo, 50		CD19	16	IIV4	823	401.2	392
Post-4	18–60	F	CLL			CD19	50	ccIIV4	334	14.4	488
Post-11	61–75	M	NHL			CD19	50	ccIIV4	189	0	501
Post-13	61–75	M	MM	Auto, 86		BCMA	14	aIIV3	290	164.7	317
Individuals without antibody responses
Post-2	18–60	F	ALL	Allo, 31		CD19	13	ccIIV4	371	0	176
Post-3	18–60	F	ALL	Allo, 60		CD19	57	IIV4	310	<0.1	152
Post-5	61–75	M	CLL			CD19	54	IIV4	217	<0.1	480
Post-6	61–75	F	CLL			CD19	21	IIV3-HD	286	2.8	332
Post-7	18–60	M	NHL			CD19	19	IIV4**	527	<0.1	394
Post-8	18–60	M	NHL			CD19	19	IIV4	416	0.9	353
Post-9	18–60	F	NHL	Auto, 48		CD19	34	IIV4	447	94.7	504
Post-10	61–75	M	NHL			CD19	24	IIV4	364	207.3	303
Post-12	61–75	M	NHL			CD19	20	IIV	324	0.3	304

Additional information is provided in online supplemental tables 1 and 2. Blank fields indicate not applicable. Baseline is defined as the day of the baseline blood sample prior to vaccination.
*Lower limit of normal; IgG, 610 mg/dL; CD19+ B cells, 100 cells/µL; CD4+ T cells, 500 cells/µL.
†All individuals in the pre-CAR-T cohort had relapsed/refractory disease at baseline. All individuals in the post-CAR-T cohort had complete remission or very good partial remission at baseline.
‡Monoclonal antibodies were: ‘pre-1’, blinatumomab; ‘pre-4’ and ‘pre-5’, daratumumab.
§CAR-T-cell protocols are depicted in online supplemental table S1.
¶Vaccine strains were: A/Brisbane/02/2018 (H1N1)pdm09-like virus, A/Kansas/14/2017 (H3N2)-like virus, B/Colorado/06/2017-like virus (B/Victoria/2/87 lineage) for IIV3, with the addition of a B/Phuket/3073/2013-like virus (B/Yamagata/16/88 lineage) for IIV4.
**One of the 13 individuals in the post-CAR-T cohort did not receive an influenza vaccine in the prior season.
aIIV3, adjuvant trivalent inactivated influenza vaccine; ALL, acute lymphoblastic leukemia; Allo, allogeneic; Auto, autologous; BCMA, B cell maturation antigen; CAR-T-cell, chimeric antigen receptor-modified T cell; CAR-Tx, CAR-T-cell therapy; cc, cell culture based; CLL, chronic lymphocytic leukemia; F, female; HCT, hematopoietic cell transplant; HD, high dose; IIV3, trivalent inactivated influenza vaccine; IIV4, quadrivalent inactivated influenza vaccine; M, male; mAb, B cell lineage targeted monoclonal antibody; MM, multiple myeloma; NHL, non-Hodgkin's lymphoma.