Binding to HER3-expressing tumor cells of scDb/scFv-Fc and scDb/Fab-Fc variants
| LIM1215 20,000 HER3/cell | MCF-7 18,000 HER3/cell | SKBR-3 14,000 HER3/cell | FaDu 3000 HER3/cell | Jurkat | |
| scDb/scFv-Fc (1-2)+1 | 31±7 | 39±5 | 29±11 | 41±40 | 1290±502 |
| scDb/Fab-Fc (1-2)+1 | 53±13 | 17±9 | 12±3 | 138±121 | 2269±846 |
| scDb/scFv-Fc (1-1)+2 | 882±588 | 390±327 | 317±257 | 879±308 | 688±189 |
| scDb/Fab-Fc (1-1)+2 | 249±192 | 711±255 | 482±371 | 258±232 | 8655±6943 |
| scDb/scFv-Fc (2-1)+1 | 82±46 | 31±4 | 33±8 | 45±37 | 1516±130 |
| scDb/Fab-Fc (2-1)+1 | 172±115 | 137±103 | 38±8 | 210±159 | 1667±223 |
EC50 (pM), mean±SD, n=3.
scDb, single-chain diabody.