Antibodies used for flow cytometry
Sr. no. | Antibody | Clone | Fluorophore |
1 | Anti-MUC1 (Novus Biologicals) | SM3 | AF700 |
2 | Anti-Her2/neu (Biolegend) | 24D2 | APC |
3 | Anti-CH2 spacer | Detection of MUC1.CAR expression | AF647 |
4 | Recombinant Erb2/Fc chimera (R&D Systems) followed by addition of the goat anti-human IgG Fc secondary antibody (SouthernBiotech) | Detection of HER2.CAR expression | AF647 |
5 | Biotinylated TR2 protein (Acro Biosystems) followed by addition of Streptavidin (Biolegend) | Detection of TR2.41BB receptor expression | BV421 |
6* | Anti-CD33 | WM53 | PE |
7 | Anti-HLA-DR | G46-6 | FITC |
8 | Anti-CD11b | ICRF44 | APC |
9 | Anti-CD14 | HCD14 | APC.Cy7 |
10 | Anti-CD15 | W6DE | BV510 |
11 | Anti-TR2 | YM366 | BV421 |
12 | Anti-CD45 | J33 | AF700 |
13 | Anti-CD3 | SK7 | PE |
14 | Anti-CD4 | 13B8.2, SK3 | Krome Orange, PE |
15 | Anti-CD8 | SFCI21Thy2D3, SK1, B9.11 | PC7, PerCP, AF700 |
16 | Anti-CD56 | NCAM16.2 | FITC |
17 | Anti-CD19 | 4G7 | PerCP |
18 | Anti-CD3 | UCHT1 | AF-AF750 |
19 | Anti-CD62L | DREG-56 | APC |
20 | Anti-CD45RA | 2H4 | PacBlue |
21 | Anti-PD1 | MIH4 | PE |
22 | Anti-TIM3 | F38-E2E | APC |
23 | Anti-41BB | 4B4-1 | BV421 |
24 | Anti-CD25 | M-A251 | PE |
25* | Anti-CD69 | L78 | PerCP |
*The above antibodies obtained from either BD Biosciences or Biolegend were used across different panels for phenotyping.