Table 1

Antibodies used for flow cytometry

Sr. no.AntibodyCloneFluorophore
1Anti-MUC1 (Novus Biologicals)SM3AF700
2Anti-Her2/neu (Biolegend)24D2APC
3Anti-CH2 spacerDetection of MUC1.CAR expressionAF647
4Recombinant Erb2/Fc chimera (R&D Systems) followed by addition of the goat anti-human IgG Fc secondary antibody (SouthernBiotech)Detection of HER2.CAR expressionAF647
5Biotinylated TR2 protein (Acro Biosystems) followed by addition of Streptavidin (Biolegend)Detection of TR2.41BB receptor expressionBV421
6*Anti-CD33WM53PE
7Anti-HLA-DRG46-6FITC
8Anti-CD11bICRF44APC
9Anti-CD14HCD14APC.Cy7
10Anti-CD15W6DEBV510
11Anti-TR2YM366BV421
12Anti-CD45J33AF700
13Anti-CD3SK7PE
14Anti-CD413B8.2, SK3Krome Orange, PE
15Anti-CD8SFCI21Thy2D3, SK1, B9.11PC7, PerCP, AF700
16Anti-CD56NCAM16.2FITC
17Anti-CD194G7PerCP
18Anti-CD3UCHT1AF-AF750
19Anti-CD62LDREG-56APC
20Anti-CD45RA2H4PacBlue
21Anti-PD1MIH4PE
22Anti-TIM3F38-E2EAPC
23Anti-41BB4B4-1BV421
24Anti-CD25M-A251PE
25*Anti-CD69L78PerCP
  • *The above antibodies obtained from either BD Biosciences or Biolegend were used across different panels for phenotyping.