Table 1

Large-scale isolation and enrichment of cytomegalovirus (CMV)-specific T cells

HDPBMCInput PBMC (109)IFNγ+cells prior selection (%)IFNγ+cells post selection (%)Recovery of IFNγ+cells (106)CD4 +IFNγ+ (%)CD8 +IFNγ+ (%)
HD 272Frozen10.5841.46931
HD 187Fresh10.8792.84752
HD 32Fresh20.5888.73667
HD 33Fresh10.2752.18111
HD 187.2Fresh11.9724.63836
HD 570Fresh11.1909.33943
HD 585Fresh11.3516.87421
HD 597Fresh10.4761.31577
HD 607Fresh11.2721.24948
Average (mean±SD)1.1±0.30.8±0.576.3±11.64.2±3.349.8±21.244.0±21.0
  • HD, human donor; IFN, interferon; PBMC, peripheral blood mononuclear cells.