Different TMB cut-off values for tissue and plasma TMB high vs low in all samples (n=143) sequenced by tumor tissue panel and lung TMB panel
| Tumor cut-off (mut/Mb) | Plasma cut-off (mut/Mb) | Both high | Both low | T-High P-Low | T-Low P-High | P-High PPA | P-High NPA | P-High PPV | P-High NPV |
| 10 | 28 | 0.21 | 0.27 | 0.50 | 0.02 | 0.30 | 0.93 | 0.91 | 0.35 |
| 12 | 33 | 0.18 | 0.29 | 0.51 | 0.01 | 0.26 | 0.95 | 0.93 | 0.37 |
| 14 | 37 | 0.17 | 0.42 | 0.41 | 0.00 | 0.29 | 1.00 | 1.00 | 0.50 |
| 16 | 42 | 0.15 | 0.52 | 0.32 | 0.01 | 0.31 | 0.99 | 0.95 | 0.62 |
| 18 | 46 | 0.12 | 0.58 | 0.27 | 0.03 | 0.30 | 0.95 | 0.81 | 0.68 |
| 20 | 51 | 0.08 | 0.66 | 0.22 | 0.04 | 0.28 | 0.94 | 0.67 | 0.75 |
| 22 | 56 | 0.08 | 0.68 | 0.22 | 0.03 | 0.26 | 0.96 | 0.73 | 0.76 |
| 24 | 60 | 0.06 | 0.72 | 0.20 | 0.02 | 0.24 | 0.97 | 0.75 | 0.79 |
The detailed patient counts in 2 by 2 matrices are represented in online supplemental table S9.
Bold values indicate a tTMB cutoff of 16 mut/Mb which was used in the further analyses.
NPA, negative per cent agreement; proportion of tissue TMB low samples called low in plasma; NPV, negative predictive value; proportion of plasma TMB low calls called low in tissue; P, TMB values in plasma; PPA, positive per cent agreement; proportion of tissue TMB high samples called high in plasma; PPV, predictive positive value ; proportion of plasma TMB high samples called high in tissue; T, TMB values in tissue; TMB, tumor mutational burden.