Table 1

Amino acid sequence of 6×His-SUMO-XCL1(CC3)-LPETGG-FLAG

FeatureAA sequence
N-terminal histagMGSSHHHHHH
SUMO solubility domain
↓: ULP-1 cleavage site
SSGLVPRGSHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLR
FLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG↓
XCL1 V21C/V59CVGSEVSDKRTCVSLTTQRLPCSRIKTYTITEGSLRAVIFITKRGLKVCADPQATWVRDCVRSMDRKSNTRNNMIQ
TKPTGTQQSTNTAVTLT
G4S – sortagging site
↓: sortase cleavage site
GGGGGSLPET↓GG
FLAG tagDYKDDDDK