Table 1

Summary table of density and spatial distribution of T cells and macrophages in each site of melanoma metastasis (liver, brain, lung, subcutaneous (subcut) and lymph node

T cell (IHC)T cell spatial distributionMyeloid (IHC)Myeloid spatial distribution
Liver∨CD3(154/mm2)
∨PD-1 (0.7%)
∧Tim-3 (10.1%)		∧CD68
Brain∨CD3 (181 /mm2)
∧PD-1 (8.9%)
∨FoxP3 (<0.5%)
∨CD103 (2.6%)

  • Lowest density of CD3+ T cells within 20 µm of a melanoma cell (78 cells/ mm2)

∨CD68
∨CD14
∨PD-L1

  • Furthest average distance between CD3+ T cells and melanoma cells (464 µm)

  • Lowest density of CD68+ macrophages within 20 µm of a melanoma cell (16 cells/mm2)

Subcut∧CD103 (14.1%),
∧FoxP3 (3.7)

  • Furthest average distance between CD3+ T cells and melanoma cells (134 µm)

Lung∧CD3 (436 /mm2)
∧CD103 (12.6%),
∧Tim-3 (12.9%)

  • Shortest average distance between CD3+ T cells and Melanoma cells (57 µm)

  • Highest density of CD3+ T cells within 20 µm of a melanoma cell (282 cells/mm2)

∧CD68
∧PD-L1

  • Shortest average distance between CD68+ macrophages and melanoma cells (47 µm)

  • Highest density of CD68+ macrophages within 20 µm of a melanoma cell (395 cells/mm2)

Lymph node∧CD3 (467 /mm2)
∧FoxP3 (3.4%)
∨Tim-3 (0.9%)

  • ∨=lowest expression, ∧=highest expression. Where two organ sites had similarly higher or lower expression of a particular marker both were included.

  • IHC, immunohistochemistry.