Table 1

Cytometry by time of flight (CyTOF) barcodes and antibodies

AntigenMassMetal conjugateCloneVendorComment
Technical channels
 Nucleic acid intercalator198PtNAFluidigmLive-dead
 DNA-1191IrNAFluidigmNuclear DNA
 DNA-2193IrNAFluidigmNuclear DNA
 BC105105Pt104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
 BC113113In104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
 BC115115In104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
 BC194194Pt104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
 BC195195Pt104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
 BC196196Pt104.2BioXcellAll hematopoietic cells except mature erythrocytes and platelets
Cell-type markers
 Ly6G141Pr1A8FluidigmGranulocytes, macrophages/monocytes
 CD11c142NdN418FluidigmT cells, DCs, NK cells, stem cells/progenitors, macrophages/monocytes, granulocytes
 CD11b143NdM1/70FluidigmDCs, NK cells, macrophages/monocytes
 CD4145NdRM-4–5FluidigmT cells, DCs, NK cells, stem cells/progenitors, macrophages/monocytes
 CD19149Sm6D5FluidigmB cells, DCs, stem cell/progenitors
 Ly6C150NdHK1.4FluidigmMacrophages, monocytes, neutrophils, T cells
 CD3152Sm145–2 C11FluidigmNKT cells, T cells, thymocytes
 NK1.1155GdPK136FluidigmNK cells, NKT cells, NKp22 cells
 CD8168Er53–6.7FluidigmMost thymocytes, T-cell subset, some NK cells, lymphoid DCs
 CD44171YbIM7FluidigmB cells, leukocytes, Tregs, T-cell memory
 MHC 2 - I-A/I-E174YbM5/114.15.2FluidigmAntigen-presenting cells, B cells, DCs, T cells, Tregs
Cell state markers and intracellular functional markers
 IL-2144NdJES65H4FluidigmT cells
 IL-12p70156GdC18.2eBioscienceB lymphocytes, DCs, and macrophages, ILC1
 Foxp3158GdFJK16sFluidigmRegulatory T cells
 IL-22160GdPoly5164ThermoFisherTh17, Th1, Th2, lymphoid tissue inducer cells, and subsets of natural killer cells, ILC
 TNF-α162DyMP6XT22ThermoFisherActivated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
 IL-17165HoTC11-18H10BioLegendCD4 memory T cells, ILC3
 IL-4166Er11B11FluidigmMast cells, T cells, bone marrow stromal cells, ILC2
 IL-6167ErMP520F3FluidigmT cells, B cells, macrophages, bone marrow stromal cells
 IFN-γ176YbXMG1.2ThermoFisherT cells, NK cells, ILC1
  • Source: BD CD Marker Handbook, BioLegend.

  • DC, dendritic cell; IFN-γ, interferon gamma; IL, interleukin; NK, natural killer; TNF-α, tumor necrosis factor alpha.