Table 1

List of SJ-600 series viruses used in this study

Virus	Descriptive name	VV gene deactivation (recombination site)	CD55 expression	VV TM used for CD55 expression	VV TM a.a. position	Marker genes
JX-594	VV-GM-CSF	J2R	No	N/A	N/A	LacZ
SJ-610	VV-GM-CSF	J2R	No	N/A	N/A	Luc-GFP
SJ-601	VV-GM-CSF-CD55 D8	J2R	Yes	D8L	276–304	Luc-GFP
SJ-604	VV-GM-CSF-CD55 A16	J2R	Yes	A16L	343–377	Luc-GFP
SJ-605	VV-GM-CSF-CD55 F9	J2R	Yes	F9L	176–212	Luc-GFP
SJ-606	VV-GM-CSF-CD55 G9	J2R	Yes	G9R	320–340	Luc-GFP
SJ-607	VV-GM-CSF-CD55 H3	J2R	Yes	H3L	285–324	Luc-GFP
SJ-608	VV-GM-CSF-CD55 L1	J2R	Yes	L1R	184–250	Luc-GFP

All viruses were derived from the Wyeth strain of vaccinia virus and all expressed GM-CSF. An expression cassette including GM-CSF was inserted into the thymidine kinase (J2R) gene of the vaccinia genome to inactivate this gene. The CD55 gene was fused with the transmembrane domain of one of the vaccinia virus membrane proteins, as indicated.
GFP, green fluorescent protein; GM-CSF, granulocyte-macrophage colony-stimulating factor; LacZ, β-galactosidase; Luc, luciferase; N/A, not applicable; TM, transmembrane domain; VV, vaccinia virus.