Figure 3

STING signaling mediates SnC activation of BMDCs. For analysis of DC activation/maturation, Cas9 STING KO and scramble control CT26 cells were treated with DMSO, veliparib, IR (12 Gy), IR+veliparib (12 Gy+20 µM), etoposide (2 µM) or GSK461364 (5 µM) for 5 days, followed by coculturing with BMDCs overnight. Then, the viable CD11c⁺/CD103⁺ DC population was analyzed for expression of CD80, CD86, H-2K^d and PD-L1. Shown are bar graphs of geometric MFI (n=3, mean±SD). Paired t-test. ***p<0.001, **0.001<p<0.01, *0.01<p<0.05, n.s. p>0.05. BMDCs, bone marrow-derived dendritic cells; DC, dendritic cell; DMSO, dimethyl sulfoxide; MFI, mean fluorescence intensity; IR, irradiation; KO, knock out; PD-L1, programmed cell death ligand 1; SnCs, senescent cells.